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Using of Tyramine Signal Amplification to Improve the Sensitivity of ELISA for Aflatoxin B1 in Edible Oil Samples

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Abstract

Tyramine signal amplification (TSA), the excellent signal amplification strategy, has the potential to improve the sensitivities of analytes analysis. In this work, the sensitivity of enzyme-linked immunosorbent assays (ELISA) has significantly improved by coupling TSA system and further biotin-streptavidin (BSA) system. Thus, a sensitive TSA-ELISA based on TSA was developed for detecting aflatoxin B1 (AFB1) in edible oil samples. Under optimal conditions, the limit of detection (LOD, IC10) and the half-maximal inhibition concentration (IC50) of the TSA-ELISA were 0.004 and 0.039 ng/mL for AFB1, respectively. The developed TSA-ELISA for AFB1 has an 11-fold improved LOD value and 6-fold improved IC50 value when compared with ELISA. The cross-reactivities of the TSA-ELISA with its analogues were negligible (< 3.48%), which indicated high specificity. The spiked recoveries were 81.4 to 118.8% with relative standard deviations (RSDs) of 3.8 to 9.0% for AFB1 in edible oil samples. Furthermore, the results of TSA-ELISA correlated well with those obtained by HPLC-fluorescence detector. The proposed TSA-ELISA was a satisfactory tool for sensitive, inexpensive, high-throughput, and alternative detection of AFB1 in edible oil samples. This study could provide the strategy for improving the sensitivity of ELISA with simple and practical approach, which has significant popularizing value and application prospect.

Comparison of the ELISA and TSA-ELISA for aflatoxin B1.

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Funding

This work was supported by the National Natural Science Foundation of China (31701687, 31570414, 31770446), the National Key Research Development Program of China (2017YFC1200100, 2016YFC0502002), the Natural Science Foundation of Jiangsu Province (BK20170537), the China Postdoctoral Science Foundation (2016 M601745), the Senior Talent Scientific Research Initial Funding Project of Jiangsu University (16JDG035), the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and the Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment.

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Author notes

  1. Yuanyuan Zhang and Ming Li contributed equally to this work.

Authors and Affiliations

  1. Institute of Environment and Ecology, Institute of Environmental Health and Ecological Security, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, People’s Republic of China

    Yuanyuan Zhang, Ming Li, Yin Cui, Xia Hong & Daolin Du

Authors
  1. Yuanyuan Zhang
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  2. Ming Li
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  3. Yin Cui
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  4. Xia Hong
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  5. Daolin Du
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Corresponding author

Correspondence to Daolin Du.

Ethics declarations

All animals used in this study and animal experiments were approved by the Committee of Laboratory Animal Management of Jiangsu Province. The license number was SYXK (SU) 2007-0005.

Ethical Approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Conflict of Interest

Yuanyuan Zhang declares that she has no conflict of interest. Ming Li declares that he has no conflict of interest. Yin Cui declares that she has no conflict of interest. Xia Hong declares that she has no conflict of interest. Daolin Du declares that he has no conflict of interest.

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Zhang, Y., Li, M., Cui, Y. et al. Using of Tyramine Signal Amplification to Improve the Sensitivity of ELISA for Aflatoxin B1 in Edible Oil Samples. Food Anal. Methods 11, 2553–2560 (2018). https://doi.org/10.1007/s12161-018-1235-9

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  • DOI: https://doi.org/10.1007/s12161-018-1235-9

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