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Extraction and Quantification of Abscisic Acid and Derivatives in Strawberry by LC-MS

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Abstract

Phytohormones are important plant components that are involved in a signaling cascade in plant development. In strawberry, the influence of abscisic acid (ABA) and its different forms [phaseic acid (PA), dihydrophaseic acid (DPA), and ABA glucose ester (ABA-GE)] on the process of fruit maturation is not yet completely understood. Quantification of phytohormones is currently performed by liquid chromatography coupled to mass spectrometry (LC-MS) due to its sensitivity and specificity. However, the sample matrix and the extraction procedure will influence the analysis. Thus, this study aimed to optimize a simple extraction method and validate the LC-MS quantification of ABA as well as the identification and quantification of ABA derivatives (PA, DPA, and ABA-GE) in strawberry fruit. Hormone extraction was performed using either methanol (80% v/v—S1) or acetone:water:acetic acid (80:19:1 v/v—S2) solutions with or without the use of sonication. The most efficient extraction was obtained using S1 without sonication, and LC-MS validation parameters for ABA were within acceptable scores.

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Funding

This study was developed with funding support by SDECT-RS, CAPES, and CNPq.

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Authors and Affiliations

  1. Programa de Pós-graduação em Ciência e Tecnologia de Alimentos, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil

    Ellen Cristina Perin, Rosane Lopes Crizel, Rafael da Silva Messias, Cesar Valmor Rombaldi & Fabio Clasen Chaves

  2. Biotecnologia, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil

    Vanessa Galli

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  1. Ellen Cristina Perin
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  2. Rosane Lopes Crizel
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  3. Vanessa Galli
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  4. Rafael da Silva Messias
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  5. Cesar Valmor Rombaldi
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  6. Fabio Clasen Chaves
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Correspondence to Fabio Clasen Chaves.

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Conflict of Interest

Ellen Cristina Perin declares that she has no conflict of interest. Rosane Lopes Crizel declares that she has no conflict of interest. Vanessa Galli declares that she has no conflict of interest. Rafael da Silva Messias declares that he has no conflict of interest. Cesar Valmor Rombaldi declares that he has no conflict of interest. Fabio Clasen Chaves declares that he has no conflict of interest.

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This article does not contain any studies with human participants performed by any of the authors.

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Perin, E.C., Crizel, R.L., Galli, V. et al. Extraction and Quantification of Abscisic Acid and Derivatives in Strawberry by LC-MS. Food Anal. Methods 11, 2547–2552 (2018). https://doi.org/10.1007/s12161-018-1224-z

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  • DOI: https://doi.org/10.1007/s12161-018-1224-z

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