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Preparation of the Cf-GQDs-Escherichia coli O157: H7 Bioprobe and Its Application in Optical Imaging and Sensing of Escherichia coli O157: H7

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Abstract

The food-borne diseases triggered by Escherichia coli O157: H7 (E. coli O157: H7) have become a life-threatening problem for tens of thousands of people around the world in the past few decades. Therefore, a rapid, simple, and user-friendly biosensing label to monitor E. coli O157: H7 has become necessary and important. In this work, carboxyl functionalized graphene quantum dots (cf-GQDs) as fluorescent labels for optical imaging and sensing in E. coli O157: H7 have been firstly proposed and evaluated. E. coli O157: H7 antibody is successfully covalently conjugated to water-soluble cf-GQDs via a cross-linking reaction, and then cf-GQDs immunofluorescence probe is achieved. The probe can effectively recognize E. coli O157: H7 and generate strong fluorescent signal. The experimental result shows that the blocking agent can obviously improve the specificity of the bioprobe for E. coli O157: H7 with the minimum detection limit of 100 cfu/mL. This novel detection method could be as a useful tool to detect E. coli O157: H7 in food, water, and environment.

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Funding

This work was funded by the National Natural Science Foundation of China (Grant no. 31300803), Program for Innovation Team Building at Institutions of Higher Education in Chongqing (Grant no. CXTDX201601030), and the Chongqing Research Program of Basic Research and Frontier Technology (Grant no. cstc2015jcyjA50034).

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Authors and Affiliations

  1. School of Science, Chongqing University of Technology, Chongqing, 400054, China

    Xiaozhan Yang & Xiang Qin

  2. Chongqing No. 8 Secondary School, Chongqing, 400030, China

    Luqiao Feng

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  1. Xiaozhan Yang
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  2. Luqiao Feng
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  3. Xiang Qin
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Correspondence to Xiaozhan Yang.

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Xiaozhan Yang declares that she has no conflict of interest. Luqiao Feng declares that she has no conflict of interest. Xiang Qin declares that he has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Informed consent was obtained from all individual participants included in the study.

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Yang, X., Feng, L. & Qin, X. Preparation of the Cf-GQDs-Escherichia coli O157: H7 Bioprobe and Its Application in Optical Imaging and Sensing of Escherichia coli O157: H7. Food Anal. Methods 11, 2280–2286 (2018). https://doi.org/10.1007/s12161-018-1207-0

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  • DOI: https://doi.org/10.1007/s12161-018-1207-0

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