Abstract
A capillary electrophoresis method coupled to contactless conductivity detection was developed for the quantitative determination of biogenic amines. Complete resolution of five biogenic amines, which are mostly found in dairy products, were performed in less than 6 min. Separation electrolyte composition was selected as 500 mM α-hydroxyisobutyric acid (HIBA) at pH 2.05. The detection limits of the method for biogenic amines were found between 0.041 and 0.098 mg/L. The precisions for the corrected peak areas were calculated as between 2.35 and 4.25% RSD. The applicability of the method was demonstrated by analyzing biogenic amines in the cheese, yoghurt, and kefir samples. The method does not require any pre-concentration and derivatization steps and can be applied as a simple, rapid, and precise method for the direct determination of biogenic amines in food samples.
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References
Al Bulushi I, Poole S, Deeth HC, Dykes GA (2009) Biogenic amines in fish: roles in intoxication, spoilage, and nitrosamine formation: a review. Crit Rev Food Sci Nutr 49(4):369–377. https://doi.org/10.1080/10408390802067514
Bardocs S (1995) Polyamines in food and their consequences for food quality and human health. Trends Food Sci Technol 6(10):341–346. https://doi.org/10.1016/S0924-2244(00)89169-4
Başkan S, Tezcan F, Köse S, Öztekin N, Erim FB (2010) Non-ionic micellar electrokinetic chromatography with laser-induced flourescence: a new method tested with biogenic amines in brined and dry-salted fish. Electrophoresis 31(13):2174–2179. https://doi.org/10.1002/elps.200900683
Benkerroum N (2016) Biogenic amines in dairy products: origin, incidence, and controlmeans. Comp Rev In Food Sci Food Saf 15(4):801–826. https://doi.org/10.1111/1541-4337.12212
Coufal P, Zuska J, van de Goor T, Smith V, Gas B (2003) Separation of twenty underivatized essential amino acids by capillary zone electrophoresis with contactless conductivity detection. Electrophoresis 24(4):671–677. https://doi.org/10.1002/elps.200390079
da Silva JAF, Guzman N, do Logo CL (2002) Contactless conductivity detection for capillary electrophoresis- Hardware improvements and optimization of the input-signal amplitude and frequency. J Chromatogr A 942(1-2):249–258. https://doi.org/10.1016/S0021-9673(01)01380-2
Erim FB (2013) Recent analytical approaches to the analysis of biogenic amines in food samples. TrAC Trends Anal Chem 52:239–247. https://doi.org/10.1016/j.trac.2013.05.018
EU Directive (2007) Amending Regulation (EC) No 2073/2005 on microbiological criteria for foodstuffs. Commission Regulation (EC) No 1441/2007 of 5 December 2007. Official Journal of European Union.http://eur-lex.europa.eu/eli/reg/2007/1441/oj/eng. Accessed 13 July 2017
Hashemi P, Erim FB (2016) Analysis of vitamin B2 in saffron stigmas (Crocus sativus L) by capillary electrophoresis coupled with laser-induced fluorescence detector. Food Anal Methods 9(8):2395–2399. https://doi.org/10.1007/s12161-016-0430-9
Kalaycıoğlu Z, Hashemi P, Günaydın K, Erim FB (2015) The sensitive capillary electrophoretic-LIF method for simultaneous determination of curcuminoids in turmeric by enhancing fluorescence intensities of molecules upon inclusion into (2-hydroxypropyl)-beta-cyclodextrin. Electrophoresis 36(20):2516–2521. https://doi.org/10.1002/elps.201500253
Kvasnička F, Voldřich M (2006) Determination of biogenic amines by capillary zone electrophoresis with conductometric detection. J Chromatogr A 1103(1):145–149. https://doi.org/10.1016/j.chroma.2005.11.005
Li W, Pan Y, Liu Y, Zhang X, Ye J, Chu Q (2014) Simultaneous determination of eight typical biogenic amines by CZE with capacitively coupled contactless conductivity detection. Chromatographia 77(3-4):287–292. https://doi.org/10.1007/s10337-013-2595-3
Linares DM, del Río B, Ladero V, Martínez N, Fernández M, Martín MC, Álvarez MA (2012) Factors influencing biogenicamines accumulation in dairy products. Front in Microbiol 3:1–10. https://doi.org/10.3389/fmicb.2012.00180
Loizzo MR, Menichini F, Picci N, Puoci F, Spizzirri UG, Restuccia D (2013) Technological aspects and analytical determination of biogenic amines in cheese. Trends Food Sci Technol 30(1):38–55. https://doi.org/10.1016/j.tifs.2012.11.005
Min JS, Lee SO, Jang A, Lee M, Kim Y (2004) Quantitative analysis of biogenic amines in raw and processed foods of animal origin on Korean domestic market. Asian-Aust J Anim Sci 17(12):1764–1768. https://doi.org/10.5713/ajas.2004.1764
Novella-Rodríguez S, Veciana-Nogués MT, Vidal-Carou MC (2000) Biogenic amines and polyamines in milks and cheeses by ion-pair high performance liquid chromatography. J Agric Food Chem 48(11):5117–5123. https://doi.org/10.1021/jf0002084
Özdestan Ö, Üren A (2010) Biogenic amine content of kefir: a fermented dairy product. Eur Food Res Technol 231(1):101–107. https://doi.org/10.1007/s00217-010-1258-y
Sentellas S, Núñez O, Saurina J (2016) Recent advances in the determination of biogenic amines in food samples by (U)HPLC. J Agric Food Chem 64(41):7667–7678. https://doi.org/10.1021/acs.jafc.6b02789
Shen D, Li Y, Zhang Z, Zhang P, Kang Q (2013) Determination of amino acids by capillary electrophoresis with differential resonant contactless conductivity detector. Talanta 104:39–43. https://doi.org/10.1016/j.talanta.2012.11.027
Strieglerová L, Kubáň P, Boček P (2011) Electromembrane extraction of amino acids from body fluids followed by capillary electrophoresis with capacitively coupled contactless conductivity detection. J Chromatogr A 1218(37):6248–6255. https://doi.org/10.1016/j.chroma.2011.07.011
Tůma P, Samcová E, Andělová K (2006) Determination of free amino acids and related compounds in amniotic fluid by capillary electrophoresis with contactless conductivity detector. J Chromatogr B 839(1-2):12–18. https://doi.org/10.1016/j.jchromb.2005.12.020
Uzaşçı S, Başkan S, Erim FB (2012) Biogenic amines in wines and pomegranate molasses- a non-ionic micellar electrokinetic chromatograph assay with laser-induced fluorescence detection. Food Anal Methods 5(1):104–108. https://doi.org/10.1007/s12161-011-9220-6
Valsamaki K, Michaelidou A, Polychroniadou A (2000) Biogenic amine production in feta cheese. Food Chem 71(2):259–266. https://doi.org/10.1016/S0308-8146(00)00168-0
Zemann AJ (2003) Capacitively coupled contactless conductivity detection in capillary electrophoresis. Electrophoresis 24(1213):2125–2137. https://doi.org/10.1002/elps.200305476
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We thank to the Research Foundation of Istanbul Technical University for financial support.
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Veselina Adımcılar declares that she has no conflict of interest.
Nevin Öztekin declares that she has no conflict of interest.
F. Bedia Erim declares that she has no conflict of interest.
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Adımcılar, V., Öztekin, N. & Bedia Erim, F. A Direct and Sensitive Analysis Method for Biogenic Amines in Dairy Products by Capillary Electrophoresis Coupled with Contactless Conductivity Detection. Food Anal. Methods 11, 1374–1379 (2018). https://doi.org/10.1007/s12161-017-1122-9
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DOI: https://doi.org/10.1007/s12161-017-1122-9