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Development of a Homologous Fluorescence Polarization Immunoassay for Diisobutyl Phthalate in Romaine Lettuce

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Abstract

In this work, three fluorescein-labeled DiBP derivatives (tracers) with different chemical structures and spacer bridges were synthesized and purified by thin-layer chromatography (TLC). Compared with the heterologous tracer, the homologous tracer exhibited more affinity to the antibody. What is more, the tracer concentration and the antibody dilution were further evaluated to improve the sensitivity of fluorescence polarization immunoassay (FPIA). On the basis of sensitive antibody and tracer, a rapid and specific FPIA has been established for the detection of DiBP contamination in the romaine lettuce, which has rarely been reported before. Under the optimal conditions, the developed FPIA showed a good detection range from 8.82 to 2152.84 ng/mL, with a detection limit of 1.77 ng/mL. In addition, the cross-reactivity to several compounds structurally related to DiBP was less than 7.37 %. Therefore, DiBP contamination in spiked romaine lettuce samples was detected by FPIA, with the recovery from 88.28 to 119.11 %. Moreover, when compared with high-performance liquid chromatography (HPLC) and enzyme-linked immunosorbent assay (ELISA), the results analyzed by the developed FPIA shows a strong reliability and a high correlation value of 0.978 and 0.980, respectively. Thus, this data, combined with rapidity and simplicity of the assay, demonstrates that the established FPIA is a suitable method for high throughput screening of DiBP contamination in the romaine lettuce.

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Acknowledgments

The authors thank Dr. Ying Zhang of the School of Foreign Languages at Guangdong University of Technology for assisting with the language editing. This work was financially supported by the National Natural Science Foundation of China (41071176 and 41271340) and Guangdong Natural Science Foundation (S2013030013338).

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Authors and Affiliations

  1. Department of Pharmaceutical Engineering, Faculty of Chemical Engineering and Light Industry/Guangdong Provincial Key Laboratory of Functional Soft Condensed Matter, Guangdong University of Technology, Guangzhou, 510006, China

    Yingshan Chen, Xiping Cui, Panpan Wu, Zhengyun Jiang, Luoying Jiao, Qingqing Hu & Suqing Zhao

  2. Department of Chemical Enzymology, Faculty of Chemistry, M.V. Lomonosov State University, Moscow, 119992, Russia

    Sergei A. Eremin

Authors
  1. Yingshan Chen
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  2. Xiping Cui
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  3. Panpan Wu
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  4. Zhengyun Jiang
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  5. Luoying Jiao
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  6. Qingqing Hu
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  7. Sergei A. Eremin
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  8. Suqing Zhao
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Corresponding author

Correspondence to Suqing Zhao.

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Conflict of Interest

Chen Yingshan declares that she has no conflict of interest. Cui Xiping declares that he has no conflict of interest. Wu Panpan declares that he has no conflict of interest. Jiang Zhengyun declares that he has no conflict of interest. Jiao Luoying declares that she has no conflict of interest. Hu Qingqing declares that she has no conflict of interest. Sergei A. Eremin declares that he has no conflict of interest. Zhao Suqing declares that he has no conflict of interest.

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All applicable international, national, and institutional guidelines for the care and use of laboratory animals were followed. This article does not contain any studies with human participants performed by any of the authors.

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Yingshan Chen and Xiping Cui contributed equally to this work and should be considered co-first authors.

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Chen, Y., Cui, X., Wu, P. et al. Development of a Homologous Fluorescence Polarization Immunoassay for Diisobutyl Phthalate in Romaine Lettuce. Food Anal. Methods 10, 449–458 (2017). https://doi.org/10.1007/s12161-016-0596-1

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  • DOI: https://doi.org/10.1007/s12161-016-0596-1

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