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Discrimination of Alicyclobacillus Strains by Lipase and Esterase Fingerprints

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Abstract

Alicyclobacillus spp. have recently become a major issue in the fruit juice industry due to its implication in the spoilage of pasteurized juices/beverages. In this study, lipase and esterase fingerprints of 37 Alicyclobacillus strains and 14 Bacillus strains in apple juice were collected using a five-substrate cocktail including four C8-esters and one C4-diester. Characterization of each strain could be finished in a single assay followed by high-performance liquid chromatography (HPLC) analysis for product separation and quantification. The obtained lipase and esterase fingerprints were highly species-dependent. Differences between the relative peak areas of each product for different species were significant. Coupled to chemometric techniques including principal component analysis (PCA) and stepwise linear discriminant analysis (SLDA), the lipase and esterase profiles led to 100 % of correct species identification for Alicyclobacillus and Bacillus strains. This approach is promising, reliable, and with good repeatability and could be used as an alternative tool to discriminate Alicyclobacillus spp. in fruit juices.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (31071550, 31171721) and National Science & Technology Support Program during the “12th Five-Year” (2012BAD31B01). We particularly thank Prof. Jean-Louis Reymond (Department of Chemistry and Biochemistry, University of Berne, Bern, Switzerland) for generously providing the five-substrate cocktail and for his valuable advice and guidance during our research.

Compliance with Ethical Standards

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Conflict of Interest

Rui Cai declares that he has no conflict of interest. Yahong Yuan declares that he has no conflict of interest. Zhouli Wang declares that he has no conflict of interest. Jun Wang declares that he has no conflict of interest. Tianli Yue declares that he has no conflict of interest. This article does not contain any studies with human or animal subjects.

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This article does not contain any studies with human participants performed by any of the authors.

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Authors and Affiliations

  1. College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Rui Cai, Yahong Yuan, Zhouli Wang, Jun Wang & Tianli Yue

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  1. Rui Cai
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  2. Yahong Yuan
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  3. Zhouli Wang
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  4. Jun Wang
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  5. Tianli Yue
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Correspondence to Tianli Yue.

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Cai, R., Yuan, Y., Wang, Z. et al. Discrimination of Alicyclobacillus Strains by Lipase and Esterase Fingerprints. Food Anal. Methods 9, 1128–1133 (2016). https://doi.org/10.1007/s12161-015-0290-8

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  • DOI: https://doi.org/10.1007/s12161-015-0290-8

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