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Liquid Chromatography–Hybrid Linear Ion Trap–High-Resolution Mass Spectrometry (LTQ-Orbitrap) Method for the Determination of Glycoalkaloids and Their Aglycons in Potato Samples

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Abstract

Potatoes produce biologically active secondary metabolites like glycoalkaloids and their aglycons, which may have both adverse and beneficial effects in the diet. A new analytical method that uses liquid chromatography–mass spectrometry (LTQ-Orbitrap) has been developed for the analysis of glycoalkaloids and their aglycons in potato samples. Two glycoalkaloids, α-solanine and α-chaconine, and two aglycons, demissidine and solasodine, were quantified in potato samples. Samples were extracted using methanol, purified on an SPE Strata C18 cartridge, and then analyzed in HPLC–mass spectrometry (LTQ-Orbitrap) with the FTMS operating in full scan at a resolving power of 30,000 (FWHM), enabling the detection and accurate mass measurement and with the ITMS mode operating in MRM (multiple reaction monitoring) for glycoalkaloids and their aglycons using the [M + H]+ ions and their optimized collision energies. After validation, the method was applied to screen different type of potatoes, and some cooking experiment were conducted.

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Abbreviations

LIT:

Linear ion trap

SPE:

Solid phase extraction

ESI:

Electrospray ionization

HESI:

Heated electrospray ionization

FWHM:

Full width at half maximum

FTMS:

Fourier transform mass spectrometry

CE:

Collision energies

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Conflict of Interest

Giovanni Caprioli declares that he has no conflict of interest. Michael G. Cahill declares that he has no conflict of interest. Kevin J. James declares that he has no conflict of interest. Sauro Vittori declares that he has no conflict of interest. This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. School of Pharmacy, University of Camerino, Via Sant’Agostino 1, 62032, Camerino, Italy

    Giovanni Caprioli & Sauro Vittori

  2. Environmental Research Institute, University College Cork, Lee Road, Cork, Ireland

    Michael G. Cahill & Kevin J. James

Authors
  1. Giovanni Caprioli
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  2. Michael G. Cahill
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  3. Sauro Vittori
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  4. Kevin J. James
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Correspondence to Giovanni Caprioli.

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Caprioli, G., Cahill, M.G., Vittori, S. et al. Liquid Chromatography–Hybrid Linear Ion Trap–High-Resolution Mass Spectrometry (LTQ-Orbitrap) Method for the Determination of Glycoalkaloids and Their Aglycons in Potato Samples. Food Anal. Methods 7, 1367–1372 (2014). https://doi.org/10.1007/s12161-013-9758-6

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  • DOI: https://doi.org/10.1007/s12161-013-9758-6

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