Abstract
A hydrophilic molecularly imprinted polymer was synthesized using acrylamide (C3H5ON) as the template and 2-acrylamido-2-methylpropane sulfonic acid as the functional monomer. This imprinted polymer was characterized by static and kinetic adsorption experiments, and results showed that it exhibited with good recognition ability and fast adsorption–desorption dynamics toward acrylamide in an aqueous environment. Using the prepared material as sorbent, a method of molecularly imprinted solid-phase extraction coupled with high-performance liquid chromatography for analysis of acrylamide in foods was developed. Under the optimized conditions, the limit of detection (S/N = 3) of this method for acrylamide was 72.0 ng/L, and the RSD for five replicate extractions of 10 μg/L acrylamide was 4.7 %. The blank potato samples spiked with acrylamide at different levels of 0.125, 0.250, and 0.50 μg/g were extracted and determined respectively by this developed method, and recoveries ranging from 91.5 to 95.1 % were obtained. Finally, commercial samples of twisted cruller and potato chip were quantitatively analyzed by this method.
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Acknowledgments
The authors are grateful for financial supports from National Natural Science Foundation of China (project no. 31071543) and the Science & Technology Project of Tai'an, China.
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Prof. Xuguang Qiao contributed equally to this work.
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Xu, L., Qiao, X., Ma, Y. et al. Preparation of a Hydrophilic Molecularly Imprinted Polymer and Its Application in Solid-Phase Extraction to Determine of Trace Acrylamide in Foods Coupled with High-Performance Liquid Chromatography. Food Anal. Methods 6, 838–844 (2013). https://doi.org/10.1007/s12161-012-9491-6
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DOI: https://doi.org/10.1007/s12161-012-9491-6