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Thermophilic Anaerobic Digestion of Arundo donax cv. Lvzhou No. 1 for Biogas Production: Structure and Functional Analysis of Microbial Communities

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Abstract

Thermophilic anaerobic digestion (TAD) is an efficient method for biogas production. In this study, the TAD of Arundo donax cv. Lvzhou No. 1 (ADL-1, a new kind of energy crop with high cold tolerance) at different growth stages was carried out, in order to investigate the relationship between microbial community structure and its function during the fermentation process. The results showed that the most optimal growth period of ADL-1 was 3 months, regarding the yield of biogas production. The TAD process lasted for 10 days with cumulative biogas and methane yields of 312.7 mL/g VS and 231 mL/g VS, respectively. The degradation rates of hemicellulose, cellulose, and lignin were 41.78%, 27.99%, and 14.46%, respectively. The high-throughput sequencing of 16S rRNA gene amplicons revealed that the most abundant bacterial phylum in TAD was Firmicutes with three dominant genera of Tepidiphilus, Sedimentibacter, and Gelria. Also, the main archaeal order was Methanomicrobiales, in which Methanoculleus and Methanosarcina were detected as dominant genera. Therefore, this article reveals the dynamic changes of structure and function of microbial communities during TAD of ADL-1, providing the theoretical basis for the development of energy crops with cold tolerance as potential biogas feedstock.

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Funding

This work was financially supported by grants from the Natural Science Foundation of China (31370146), Fujian Agriculture and Forestry University International Cooperation and Exchange Project (No. KXG15001A), Sub Project of National Science and Technology Support Program (2014BAD15B01-6), and Key Research and Development Plan of Jiangxi Province (20171ACF60005).

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  1. Yating Lei and Chao Xie contributed equally to this work.

Authors and Affiliations

  1. College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China

    Yating Lei, Chao Xie & Xiaoxi Wang

  2. National Engineering Research Center of JUNCAO Technology, Fuzhou, 350002, Fujian, China

    Yating Lei, Chao Xie, Xiaoxi Wang & Bin Liu

  3. College of Mechanical and Electronic Engineering, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China

    Zhiyi Fang

  4. College of Food Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China

    Ying Huang & Bin Liu

  5. College of Material Engineering, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China

    Shilin Cao

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Lei, Y., Xie, C., Wang, X. et al. Thermophilic Anaerobic Digestion of Arundo donax cv. Lvzhou No. 1 for Biogas Production: Structure and Functional Analysis of Microbial Communities. Bioenerg. Res. 13, 866–877 (2020). https://doi.org/10.1007/s12155-020-10105-y

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