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Cellulolytic Microflora Pretreatment Increases the Efficiency of Anaerobic Co-digestion of Rice Straw and Pig Manure

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Abstract

Agricultural wastes have severely polluted the environment and obstruct the sustainable development of modern agriculture due to a lack of effective disposal methods. The present study conducted batch experiments in which rice straw (RS) and pig manure (PM) mixtures were pretreated with a previously developed cellulolytic microflora prior to their mesophilic anaerobic co-digestion. Optimal anaerobic digestion (AD) performance of RS and PM could be achieved after biological pretreatment with this specific cellulolytic microflora for 30 h. Under this condition, the filter paper cellulase (FPase) and carboxymethyl cellulase (CMCase) activities in RS and PM degradation broths reached maxima of 2.25 and 2.58 IU/mL, respectively, and the weight loss ratio reached 39.4%. After the subsequent AD process, the methane yield of RS and PM mixtures reached 263.69 mL/g-VS, which was 47.6% higher than that of the control group (CK) without biological pretreatment (178.66 mL/g-VS). In addition, the daily methane production peak duration (3 day) of the anaerobic co-digestion of RS and PM after 30 h of biological pretreatment with this microflora was longer than that of CK (1 day). The above results further indicated that pretreatment of RS and PM mixtures with microflora greatly enhanced the methane yield and prolonged the peak period of methane production in the subsequent anaerobic co-digestion.

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Funding

This work was financially supported by the Outstanding Youth Talent Funding Program of Jiangxi Province (Grant No. 20171BCB23044), Science and Technology Project Founded by the Education Department of Jiangxi Province (GJJ180174), the National Natural Science Foundation of China (Grant No. 31260024) and the Science and Technology Support Program of Jiangxi Province, China (Grant No. 20151BBF60029).

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Authors and Affiliations

  1. Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, 330045, People’s Republic of China

    Fei Shen, Bin Zhong, Yanling Wang, Xiang Xia, Zhijun Zhai & Qinghua Zhang

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  1. Fei Shen
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  2. Bin Zhong
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Correspondence to Qinghua Zhang.

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Shen, F., Zhong, B., Wang, Y. et al. Cellulolytic Microflora Pretreatment Increases the Efficiency of Anaerobic Co-digestion of Rice Straw and Pig Manure. Bioenerg. Res. 12, 703–713 (2019). https://doi.org/10.1007/s12155-019-10013-w

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