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NMR assignment of Cdc42(T35A), an active Switch I mutant of Cdc42

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Abstract

Cdc42(T35A) is an active construct of Cdc42, a Ras GTPase involved in signal transduction, containing a single-point mutation in an important effector-binding region. We determined the backbone and side chain resonance assignments of 13C,15N-labelled Cdc42(T35A) from E. coli.

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Acknowledgements

The authors thank Drs. Robert McFeeters, Adriene Loh, Ahmed Ahmed, TKS Kumar, and Roger Koeppe II for helpful discussions. We also thank Mrs. Shatara Porchia-White for technical assistance.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, University of Arkansas-Fayetteville, Chemistry Building 119, Fayetteville, AR, 72701, USA

    Paul D. Adams

  2. Department of Molecular Medicine, Cornell University College of Veterinary Medicine, Ithaca, NY, USA

    Robert E. Oswald

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  1. Paul D. Adams
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  2. Robert E. Oswald
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Correspondence to Paul D. Adams.

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Adams, P.D., Oswald, R.E. NMR assignment of Cdc42(T35A), an active Switch I mutant of Cdc42. Biomol NMR Assign 1, 225–227 (2007). https://doi.org/10.1007/s12104-007-9062-8

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  • DOI: https://doi.org/10.1007/s12104-007-9062-8

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