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miR-448 negatively regulates ovarian cancer cell growth and metastasis by targeting CXCL12

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Abstract

Objectives

The purpose of the study is to investigate the roles of miR-448 in ovarian cancer.

Methods

miR-448 and CXCL12 mRNA expression were examined using qRT-PCR. CXCL12 promoter activity was detected by luciferase activity system. Cell proliferation was assayed by MTT or colony formation. Migration and invasion was assayed by transwell chamber.

Results

miR-448 expression was usually under-expressed in ovarian cancer tissues and cell lines compared with their normal ones. Ectopic expression of miR-448 inhibited cell proliferation, migration and invasion in ovarian cancer cells. Moreover, bioinformatic prediction suggested that CXCL12 was a target gene of miR-448. We also demonstrated that restored expression of CXCL12 dampened miR-448-mediated suppression of tumor progression, which suggests the important role of miR-448 in tumor progression.

Conclusion

Our data indicate that miR-448 functions as a tumor suppressor in ovarian cancer, which exerts its activity by suppressing the expression of CXCL12.

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Ethical Standards

For the use of clinical materials for research purposes, prior patients’ consents and approval were obtained from the Shiyan Taihe Hospital, Hubei University of Medcine. All samples were collected and analyzed with prior written informed consents from the patients.

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Authors and Affiliations

  1. Department of Obstetrics, Shiyan Taihe Hospital, Hubei University of Medcine, 32 Renmin South Road, Shiyan, Hubei, China

    Y. Lv, Y. Lei, Y. Hu & W. Ding

  2. Department of Gynaecology, Shiyan Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China

    C. Zhang & C. Fang

Authors
  1. Y. Lv
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  2. Y. Lei
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  3. Y. Hu
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  4. W. Ding
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  5. C. Zhang
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  6. C. Fang
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Corresponding author

Correspondence to Y. Lv.

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Lv, Y., Lei, Y., Hu, Y. et al. miR-448 negatively regulates ovarian cancer cell growth and metastasis by targeting CXCL12. Clin Transl Oncol 17, 903–909 (2015). https://doi.org/10.1007/s12094-015-1325-8

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  • DOI: https://doi.org/10.1007/s12094-015-1325-8

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