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Lack of correlation between DNA copy number and mRNA expression levels ofc-myc in γ-radiation-induced mouse thymic lymphomas by using quantitative real-time PCR

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Abstract

Background

It is well documented that over-expression of thec-myc proto-oncogene occurs in the vast majority of mouse thymic lymphomas induced by γ-irradiation, evidencing the importance of this gene in T-cell lymphomagenesis. However, it remains unknown whether elevated levels ofc-myc expression are driven by extrac-myc copy numbers.

Materials and methods

Here we use a quantitative test on the basis of real-time PCR to determine the cellular copy number of c-myc in a set of 14 g-radiation-induced thymic lymphomas obtained from (C57BL/6J x BALB/cJ) F1 hybrid mice with increased mRNA c-myc expression.

Results

Since 5 out of 14 (35.7%) cases had no extra copy numbers of c-myc, gene amplification was obviously not the cause of c-myc over-expression in these tumours. In the remaining 9 tumours, c-myc over-expression was also accompanied with extra DNA copy numbers. Therefore, c-myc amplification might be a consequence of the genomic instability subsequent to the up-regulation of c-myc. However, linear regression analysis showed a lack of correlation between increasing DNA copy numbers and mRNA over expression of c-myc in these tumours (r =0.029, p=0.94).

Conclusion

De-regulation of c-myc does not necessarity imply amplification of this gene in these tumours. This report is, to our knowledge, the first one comparing c-myc amplification with expression in lymphomas of the T-cell lineage.

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Authors and Affiliations

  1. Departamento de Biología, Laboratorio de Genética Molecular Humana. Facultad de Ciencias, Universidad Autónoma de Madrid, Crta. de Colmenar Viejo, Km. 15, Campus de Canto Blanco, 28049, Madrid, Spain

    Javier Santos, Concepción Vaquero, Janet Reyes, Pilar López-Nieva, María Matabuena, María Villa, Pablo Fernández & José Fernández-Piqueras

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  1. Javier Santos
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  2. Concepción Vaquero
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  3. Janet Reyes
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  4. Pilar López-Nieva
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  5. María Matabuena
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  6. María Villa
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  7. Pablo Fernández
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  8. José Fernández-Piqueras
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Correspondence to Javier Santos.

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Santos, J., Vaquero, C., Reyes, J. et al. Lack of correlation between DNA copy number and mRNA expression levels ofc-myc in γ-radiation-induced mouse thymic lymphomas by using quantitative real-time PCR. Clin Transl Oncol 8, 349–353 (2006). https://doi.org/10.1007/s12094-006-0181-y

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  • DOI: https://doi.org/10.1007/s12094-006-0181-y

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