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Synthesis of Protein-Inorganic Nanohybrids with Improved Catalytic Properties Using Co3(PO4)2

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Abstract

In the present study, a method for easy and rapid synthesis of lipase nanohybrids was evaluated using cobalt chloride as an encapsulating agent. The synthesized nanohybrids exhibited higher activity (181%) compared to free lipase and improved catalytic properties at higher temperature and in harsh conditions. The nanohybrids retained 84% of their residual activity at 25 °C after 10 days. In addition, these nanohybrids also exhibited high storage stability and reusability. Collectively, the synthesis of carrier-free immobilized biocatalysts was performed rapidly within 24 h at 4 °C. Their high reusability and catalytic activities highlight the broad applicability of this method for catalysis in organic and aqueous media.

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Acknowledgements

This work was supported by the 2017 KU Brain Pool Fellowship of Konkuk University. This research was supported by the Ministry of Science, ICT and Future Planning, Republic of Korea (NRF-2013R1A1A2012159 and NRF-2013R1A1A2007561). This work was also supported by the Energy Efficiency and Resources Core Technology Program of the KETEP, granted financial resource from the Ministry of Trade, Industry and Energy, Republic of Korea (20153010092130). This research was supported by the KU Research Professor program of Konkuk University.

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  1. Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul, 05029, Republic of Korea

    Ashok Kumar, In-Won Kim, Sanjay K. S. Patel & Jung-Kul Lee

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  1. Ashok Kumar
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  2. In-Won Kim
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  3. Sanjay K. S. Patel
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Correspondence to Sanjay K. S. Patel or Jung-Kul Lee.

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Kumar, A., Kim, IW., Patel, S.K.S. et al. Synthesis of Protein-Inorganic Nanohybrids with Improved Catalytic Properties Using Co3(PO4)2. Indian J Microbiol 58, 100–104 (2018). https://doi.org/10.1007/s12088-017-0700-2

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  • DOI: https://doi.org/10.1007/s12088-017-0700-2

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