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Preparation, Characterization, and Antimicrobial Properties of Chitosan–Silver Nanocomposites Films Against Fish Pathogenic Bacteria and Fungi

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Abstract

Development of nanostructured films using natural polymers and metals has become a considerable interest in various biomedical applications. Objective of the present study was to develop silver nano particles (AgNPs) embedded chitosan films with antimicrobial properties. Based on the Ag content, two types of chitosan silver nano films, named as CAgNfs-12 (12 mM) and CAgNfs-52 (52 mM) were prepared and characterized. Field emission scanning electron microscope (FE-SEM) images of two CAgNfs showed the circular AgNPs, which were uniformly embedded and distributed in the matrix of chitosan films. Antimicrobial experiment results clearly indicated that CAgNfs can inhibit the growth of fish pathogenic bacteria Vibrio (Allivibrio) salmonicida, V. tapetis, Edwardsiella tarda and fungi Fusarium oxysporum. Moreover, CAgNfs significantly reduced the experimentally exposed V. salmonicida levels in artificial seawater, suggesting that these CAgNfs could be used to develop antimicrobial filters/membranes for water purifying units to eliminate the pathogenic microbes.

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References

  1. Bozanic DK, Trandafilovic LV, Luyt AS, Djokovic V (2010) ‘Green’ synthesis and optical properties of silver–chitosan complexes and nanocomposites. React Funct Polym 70:869–873. doi:10.1016/j.reactfunctpolym.2010.08.001

    Article  CAS  Google Scholar 

  2. Lu S, Gao W, Gu HY (2008) Construction, application and biosafety of silver nanocrystalline chitosan wound dressing. Burns 34:623–628. doi:10.1016/j.burns.2007.08.020

    Article  PubMed  Google Scholar 

  3. Shen XL, Wu JM, Chen Y, Zha G (2010) Antimicrobial and physical properties of sweet potato starch films incorporated with potassium sorbate or chitosan. Food Hydrocoll. 24:285–290. doi:10.1016/j.foodhyd.2009.10.003

    Article  CAS  Google Scholar 

  4. Sharma S, Sanpui P, Chattopadhyay A, Ghosh SS (2012) Fabrication of antibacterial silver nanoparticle–sodium alginate–chitosan composite films. RSC Adv 2:5837–5843. doi:10.1039/C2RA00006G

    Article  CAS  Google Scholar 

  5. Cooper A, Oldinski R, Ma H, Bryers JD, Zhang M (2013) Chitosan-based nanofibrous membranes for antibacterial filter applications. Carbohydr Polym 92:254–259. doi:10.1016/j.carbpol.2012.08.114

    Article  CAS  PubMed  Google Scholar 

  6. Velmurugan P, Lydroose M, Lee SM, Cho M, Park JH, Balachandar V, Oh BT (2014) Synthesis of silver and gold nanoparticles using cashew nut shell liquid and its antibacterial activity against fish pathogens. Indian J Microbiol 54(2):196–202. doi:10.1007/s12088-013-0437-5

    Article  CAS  PubMed  Google Scholar 

  7. Bose D, Chatterjee S (2015) antibacterial activity of green synthesized silver nanoparticles using Vasaka (Justicia adhatoda L.) leaf extract, Indian. J Microbiol 55(2):163–167. doi:10.1007/s12088-015-0512-1

    CAS  Google Scholar 

  8. Bozanic DK, Brankovi SD, Bibic N, Luyt AS, Djokovic V (2011) Silver nanoparticles encapsulated in glycogen biopolymer: morphology, optical and antimicrobial properties. Carbohydr Polym 83:883–890. doi:10.1016/j.carbpol.2010.08.070

    Article  CAS  Google Scholar 

  9. Huang H, Yang X (2004) Synthesis of chitosan-stabilized gold nanoparticles in the absence/presence of tripolyphosphate. Biomacromol 5:2340–2346. doi:10.1021/bm0497116

    Article  CAS  Google Scholar 

  10. Guo Y, Yan H (2008) Preparation and characterization of heparin-stabilized gold nanoparticles. J Carbohydr Chem 27:309–319. doi:10.1080/07328300802158752

    Article  CAS  Google Scholar 

  11. Vigneshwaran N, Nachane RP, Balasubramanya RH, Varadarajan PV (2006) A novel one-pot ‘green’ synthesis of stable silver nanoparticles using soluble starch. Carbohydr Res 341:2012–2018. doi:10.1016/j.carres.2006.04.042

    Article  CAS  PubMed  Google Scholar 

  12. Anastas PT, Williamson TC (1998) Frontiers in green chemistry. In: Anastas PT, Williamson TC (eds) Green chemistry: frontiers in benign chemical syntheses and processes. Oxford University Press, USA

  13. Liu S, He J, Xue J, Ding W (2009) Efficient fabrication of transparent antimicrobial poly (vinyl alcohol) thin films. J Nanopart Res 11:553–560. doi:10.1007/s11051-007-9321-8

    Article  CAS  Google Scholar 

  14. Arockianathan PM, Sekara S, Kumaran B, Sastrya TP (2012) Preparation, characterization and evaluation of biocomposite films containing chitosan and sago starch impregnated with silver nanoparticles. Int J Biol Macromol 50:939–946. doi:10.1016/j.ijbiomac.2012.02.022

    Article  PubMed  Google Scholar 

  15. Rujitanaroj PO, Pimpha N, Supaphol P (2008) Wound-dressing materials with antibacterial activity from electrospun gelatin fiber mats containing silver nanoparticles. Polymer 49:4723–4732. doi:10.1016/j.polymer.2008.08.021

    Article  CAS  Google Scholar 

  16. Regiel A, Irusta S, Kyzioł A, Arruebo M, Santamaria J (2013) Preparation and characterization of chitosan–silver nanocomposite films and their antibacterial activity against Staphylococcus aureus. Nanotechnology 24:015101. doi:10.1088/0957-4484/24/1/015101

    Article  PubMed  Google Scholar 

  17. Abdelgawad AM, Hudson SM, Rojas OJ (2014) Antimicrobial wound dressing nanofiber mats from multicomponent (chitosan/silver-NPs/polyvinyl alcohol) systems. Carbohydr Polym 100:166–178. doi:10.1016/j.carbpol.2012.12.043

    Article  CAS  PubMed  Google Scholar 

  18. Sonawane S, Jacob B, Mallick R, Mohanty S, Jena P (2012) Toxicity and antibacterial assessment of chitosan coated silver nanoparticles on human pathogens and macrophage cells. Int J Nanomed 7:1805–1818. doi:10.2147/IJN.S28077

    Article  Google Scholar 

  19. Du WL, Niu SS, Xu XL, Xu ZR, Fan CL (2009) Antibacterial activity of chitosan tripolyphosphate nanoparticles loaded with various metal ions. Carbohydr Polym 75:385–389. doi:10.1016/j.carbpol.2008.07.039

    Article  CAS  Google Scholar 

  20. Yoksan R, Chirachanchai S (2010) Silver nanoparticle-loaded chitosan–starch based films: fabrication and evaluation of tensile, barrier and antimicrobial properties. Mater Sci Eng C 30:891–897. doi:10.1016/j.msec.2010.04.004

    Article  CAS  Google Scholar 

  21. Ahmad MB, Lim JJ, Shaameli K, Ibrahim NA, Tay MY (2011) Synthesis of silver nanoparticles in chitosan, gelatin and chitosan/gelatin bio nanocomposites by a chemical reducing agent and their characterization. Molecules 16:7237–7248. doi:10.3390/molecules16097237

    Article  Google Scholar 

  22. Sautour M, Edel-Hermann V, Steinberg C, Sixt N, Laurent J, Dalle F et al (2012) Fusarium species recovered from the water distribution system of a French university hospital. Int J Hyg Environ Health 215:286–292. doi:10.1016/j.ijheh.2011.11.003

    Article  PubMed  Google Scholar 

  23. Gromadzka K, Waskiewicz A, Swietlik J, Bocianowski J, Golinski P (2015) The role of wastewater treatment in reducing pollution of surface waters with zearalenone. Arch Ind Hyg Toxicol 66:159–164. doi:10.1515/aiht-2015-66-2606

    CAS  Google Scholar 

  24. Maida CM, Milici ME, Trovato L, Oliveri S, Amodio E, Spreghini E, Scalise G, Barchiesi F (2008) Evaluation of the disk diffusion method compared to the microdilution method in susceptibility testing of anidulafungin against filamentous fungi. J Clin Microbiol 46:4071–4074. doi:10.1128/JCM.01088-08

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Rhim JW, Hong SI, Park HM, Ng PKW (2006) Preparation and characterization of chitosan-based nanocomposite films with antimicrobial activity. J Agric Food Chem 54:5814–5822. doi:10.1021/jf060658h

    Article  CAS  PubMed  Google Scholar 

  26. Mihai AL, Tanase EE, Popa ME (2015) Comparative in vitro study of the chitosan application method effect on Aspergillus brasiliensis growth. Romanian Biotechnol Lett 20:10751–10757

    Google Scholar 

  27. Sarasam A, Madihally S (2005) Characterization of chitosan–polycaprolactone blends for tissue engineering applications. Biomaterials 26:5500–5508. doi:10.1016/j.biomaterials.2005.01.071

    Article  CAS  PubMed  Google Scholar 

  28. Ramaseshan R, Sundararajan S, Jose R (2007) Nanostructured ceramics by electrospinning. J Appl Phys 102:111101. doi:10.1063/1.2815499

    Article  Google Scholar 

  29. Son B, Yeom BY, Song SH, Lee CS, Hwang TS (2009) Antibacterial electrospun chitosan/poly(vinyl alcohol) nanofibers containing silver nitrate and titanium dioxide. J Appl Polym Sci 111:2892–2899. doi:10.1002/app.29233

    Article  CAS  Google Scholar 

  30. Yuan J, Gang J, Xing Z, Shen J, Kang IK, Byun H (2010) Electrospinning of antibacterial poly (vinylidene fluoride) nanofibers containing silver nanoparticles. J Appl Pol Sci 116:668–672. doi:10.1002/app.31632

    CAS  Google Scholar 

  31. Prema P, Thangaoandiyan S (2013) In-Vitro antibacterial activity of gold nanoparticles capped with polysaccharide stabilizing agents. Int J Pharm Pharm Sci 5:310–314

    CAS  Google Scholar 

  32. Ignatov M, Manolova N, Rashkov I (2007) Novel antibacterial fibers of quaternized chitosan and poly (vinyl pyrrolidone) prepared by electro spinning. Eur Polym J 43:1112–1122. doi:10.1016/j.eurpolymj.2007.01.012

    Article  Google Scholar 

  33. Lok CN, Ho CM, Chen R, He QY, Yu WY, Sun H et al (2007) Silver nanoparticles: partial oxidation and antibacterial activities. J Biol Inorg Chem 12:527–534. doi:10.1007/s00775-007-0208-z

    Article  CAS  PubMed  Google Scholar 

  34. Duran N, Marcato PD, De Conti R, Alves OL, Costa FTM, Brocchi M (2010) Potential use of silver nanoparticles on pathogenic bacteria, their toxicity and possible mechanisms of action. J Braz Chem Soc 21:949–959. doi:10.1590/S0103-50532010000600002

    Article  CAS  Google Scholar 

  35. Dakal TC, Kumar A, Majumdar RS, Yadav V (2016) Mechanistic basis of antimicrobial actions of silver nanoparticles Front Microbiol 7:1831. doi:10.3389/fmicb.2016.01831

    PubMed  Google Scholar 

  36. Hsueh YH, Lin KS, Ke WJ, Hsieh CT, Chiang CL, Tzou DY, Liu ST (2015) The Antimicrobial properties of silver nanoparticles in Bacillus subtilis are mediated by released Ag+ ions. PLoS ONE 10:e0144306. doi:10.1371/journal.pone.0144306

    Article  PubMed  PubMed Central  Google Scholar 

  37. Belluco S, Losasso C, Patuzzi I, Rigo L, Conficoni D, Gallocchio F, Cibin V, Catellani P, Segato S, Ricci A (2016) Silver as antibacterial toward Listeria monocytogenes. Front Microbiol 7:307. doi:10.3389/fmicb.2016.00307

    Article  PubMed  PubMed Central  Google Scholar 

  38. Matsumura Y, Yoshikata K, Kunisaki S, Tsuchido T (2003) Mode of bactericidal action of silver zeolite and its comparison with that of silver nitrate. Appl Environ Microbiol 69:4278–4281. doi:10.1128/AEM.69.7.4278-4281.2003

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  39. Feng QL, Wu J, Chen GQ, Cui FZ, Kim TN, Kim JO (2000) A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. J Biomed Mater Res 52:662–668. doi:10.1002/1097-4636(20001215)52:4<662:AID-JBM10>3.0.CO;2-3

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (2014R1A2A1A11054585) and part of the project titled ‘Development of Fish Vaccines and Human Resource Training’, funded by the Ministry of Oceans and Fisheries, Republic of Korea.

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Authors and Affiliations

  1. College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungnam National University, Daejeon, 34134, Republic of Korea

    S. H. S. Dananjaya, D. C. M. Kulatunga & Mahanama De Zoysa

  2. Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju City, Jeju Self-Governing Province, 63243, Republic of Korea

    G. I. Godahewa, Chamilani Nikapitiya & Jehee Lee

  3. Fish Vaccine Research Center, Jeju National University, Jeju City, Jeju Self-Governing Province, 63243, Republic of Korea

    Chamilani Nikapitiya, Jehee Lee & Mahanama De Zoysa

  4. Jeju International Marine Science Research and Education Center, Korea Institute of Ocean Science and Technology, Jeju City, Jeju Self-Governing Province, 63349, Republic of Korea

    Chulhong Oh

  5. Department of Chemistry, Faculty of Science, University of Ruhuna, Matara, Sri Lanka

    Madurani Edussuriya

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  1. S. H. S. Dananjaya
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Correspondence to Jehee Lee or Mahanama De Zoysa.

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Dananjaya, S.H.S., Kulatunga, D.C.M., Godahewa, G.I. et al. Preparation, Characterization, and Antimicrobial Properties of Chitosan–Silver Nanocomposites Films Against Fish Pathogenic Bacteria and Fungi. Indian J Microbiol 57, 427–437 (2017). https://doi.org/10.1007/s12088-017-0670-4

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