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Ultra-High Efficient Colony PCR for High Throughput Screening of Bacterial Genes

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Abstract

Current colony PCR methods are not suitable for screening genes encoded in genomic DNA and are limited to E. coli host strains. Here, we describe an ultra-high efficient colony PCR method for high throughput screening of bacterial genes embedded in the genomic DNA of any bacterial species. This new technique expands colony PCR method to several hosts as well as offers a rapid, less expensive and reliable bacterial genomic DNA extraction.

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Acknowledgements

This research was financially supported by the “Global accompanied growth R&BD program (N042600010)” through the Ministry of Trade, Industry and Energy (MOTIE, Korea).

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Author notes

  1. Mohammad Abu Hena Mostofa Jamal and Satya Priya Sharma have contributed equally to this work.

Authors and Affiliations

  1. Department of Biomedical Sciences and Institute for Medical Science, Chonbuk National University Medical School, Chonbuk National University, Jeonju, South Korea

    Mohammad Abu Hena Mostofa Jamal, Satya Priya Sharma, Hea-Jong Chung & Seong-Tshool Hong

  2. JINIS BDRD Institute, JINIS Biopharmaceuticals Co., Wanju, Chonbuk, Korea

    Hyeon-Jin Kim

  3. Department of Anatomic Pathology, School of Medicine, Kangwon National University Hospital, Kanwong National University, Chuncheon, Gangwon, 24341, South Korea

    Seungkoo Lee

  4. Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia, Bangladesh

    Mohammad Abu Hena Mostofa Jamal

  5. Department of Microbiology, Seonam University Medical School, Namwon, Chonbuk, 55321, South Korea

    Hea-Jong Chung

Authors
  1. Mohammad Abu Hena Mostofa Jamal
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  2. Satya Priya Sharma
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  3. Hea-Jong Chung
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  4. Hyeon-Jin Kim
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  5. Seong-Tshool Hong
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Correspondence to Seungkoo Lee.

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Jamal, M.A.H.M., Sharma, S.P., Chung, HJ. et al. Ultra-High Efficient Colony PCR for High Throughput Screening of Bacterial Genes. Indian J Microbiol 57, 365–369 (2017). https://doi.org/10.1007/s12088-017-0665-1

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  • DOI: https://doi.org/10.1007/s12088-017-0665-1

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