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Thermus parvatiensis RLT sp. nov., Isolated from a Hot Water Spring, Located Atop the Himalayan Ranges at Manikaran, India

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Abstract

A Gram negative, yellow pigmented, rod shaped bacterium designated as RLT was isolated from a hot water spring (90–98 °C) located at Manikaran in Northern India. The isolate grows at 60–80 °C (optimum, 70 °C) and at pH 7.0–9.0 (optimum pH 7.2). Phylogenetic analysis of 16S rRNA gene sequences and levels of DNA–DNA relatedness together indicate that the new isolate represents a novel species of the genus Thermus with closest affinity to Thermus thermophilus HB8T (99.5 %) followed by Thermus arciformis (96.4 %). A comparative analysis of partial sequences of housekeeping genes (HKG) further revealed that strain RLT is a novel species belonging to the genus Thermus. The melting G+C content of strain RLT was calculated as 68.7 mol%. The DNA–DNA relatedness value of strain RLT with its nearest neighbours (>97 %) was found to be less than 70 % indicating that strain RLT represents a novel species of the genus Thermus. MK-8 was the predominant respiratory quinone. The presence of characteristic phospholipid and glycolipid further confirmed that strain RLT belongs to the genus Thermus. The predominant fatty acids of strain RLT were iso-C17:0 (23.67 %) and iso-C15:0 (24.50 %). The results obtained after DNA–DNA hybridization, biochemical and physiological tests clearly distinguished strain RLT from its closely related species. Thus, strain RLT represents a novel species of the genus Thermus for which the name Thermus parvatiensis is proposed (=DSM 21745T= MTCC 8932T).

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Acknowledgments

This research was supported by funds from Department of Biotechnology (DBT), National Bureau of Agriculturally Important Microorganisms (NBAIM, ICAR), DU/DST-PURSE Grant, Government of India. KK, VD, SKG, RK, CT, PL, NN, AKS, RK, AN, NG gratefully acknowledge University Grants Commission (UGC), Council for Scientific and Industrial Research (CSIR), Department of Biotechnology (DBT) and Indian Council for Agricultural Research (ICAR) for providing research fellowships. We thank SAIF-DST (Sophisticated Analytical instrumentation Facility, Department of Science and Technology, Department of Anatomy, AIIMS) for providing the transmission electron facility. We also thank Dr. J. P. Euzeby (Ecole Nationale Veterinaire, Toulouse, France) for etymological advice.

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The authors state that they have no conflict of interest.

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    Authors and Affiliations

    1. Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi, 110007, India

      Vatsala Dwivedi, Kirti Kumari, Sanjay Kumar Gupta, Rekha Kumari, Charu Tripathi, Pushp Lata, Neha Niharika, Amit Kumar Singh, Roshan Kumar, Aeshna Nigam, Nidhi Garg & Rup Lal

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    Correspondence to Rup Lal.

    Additional information

    Vatsala Dwivedi and Kirti Kumari have contributed equally to this work.

    Sequence Deposited The GenBank accession number for 16S rRNA gene sequence of strain RLT (=MTCC 8932T= DSM 21745T) is EU017402.

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    Dwivedi, V., Kumari, K., Gupta, S.K. et al. Thermus parvatiensis RLT sp. nov., Isolated from a Hot Water Spring, Located Atop the Himalayan Ranges at Manikaran, India. Indian J Microbiol 55, 357–365 (2015). https://doi.org/10.1007/s12088-015-0538-4

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