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Metabolism Dependent Chemotaxis of Pseudomonas aeruginosa N1 Towards Anionic Detergent Sodium Dodecyl Sulfate

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Abstract

Sodium dodecyl sulfate (SDS) is one of the most commonly used detergent, which exhibits excellent biocidal activity against various bacteria and fungi. It is commonly employed in many detergent formulations and is employed for disinfection purposes. It is shown to be toxic to fishes, aquatic animals and is also inhibitory to microbes and cyanobacteria. We had isolated a strain belonging to Pseudomonas aeruginosa N1, from a detergent contaminated pond situated in Varanasi city India, which was able to degrade and metabolize SDS as a source of carbon. In the present investigation, we have studied chemotactic response of this strain towards SDS. The results clearly indicate that this strain showed chemotactic response towards SDS. The nature of chemotaxis was found to be metabolism dependent as glucose grown cells showed a delayed chemotactic response towards SDS. This is first study that reported chemotaxis response for P. aeruginosa towards anionic detergent SDS.

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Abbreviations

SDS:

Sodium dodecyl sulfate

PBM:

Phosphate buffered medium

TTC:

2,3,5-Triphenyl tetrazolium chloride

NACs:

Nitro aromatic compounds

2,4-D:

2,4-Dichloro phenoxy acetic acid

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Acknowledgments

V.C. is grateful to Council for Scientific and Industrial Research (CSIR), New Delhi, India for the award of Junior Research Fellowship. This study was partly supported by a research grant sanctioned to A.K. by the Department of Biotechnology, Govt. of India, New Delhi (BT/PR1239/AGR./02/065/98).

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Authors and Affiliations

  1. School of Biotechnology, Banaras Hindu University, Varanasi, 221 005, India

    Venkatesh Chaturvedi & Ashok Kumar

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  1. Venkatesh Chaturvedi
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  2. Ashok Kumar
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Corresponding author

Correspondence to Venkatesh Chaturvedi.

Electronic supplementary material

Below is the link to the electronic supplementary material.

12088_2013_426_MOESM1_ESM.tif

NATIVE PAGE Zymography of alkyl sulfatase of P. aeruginosa strain N1.Lane1. SDS grown cells, Lane 2. Glucose grown cells (TIFF 192 kb)

12088_2013_426_MOESM2_ESM.tif

Swarm Plate assay for chemotaxis. (A) Chemotaxis in presence of Glucose (Control), (B) Chemotaxis in presence of SDS. (TIFF 804 kb)

12088_2013_426_MOESM3_ESM.tif

Drop plate assay for bacterial chemotaxis. (A) chemotactic response towards Glucose,(B) SDS (10 mg), (C) SDS (30 mg), (D) response of SDS grown cells towards glucose, (E) response of glucose grown cells towards SDS, (F) response of SDS grown cells towards glucose and SDS, (G) response towards 1-dodecanol, (H) response of heat killed cells toward glucose(negative control), (I) response of heat killed cells towards SDS (negative control). (TIFF 2171 kb)

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Chaturvedi, V., Kumar, A. Metabolism Dependent Chemotaxis of Pseudomonas aeruginosa N1 Towards Anionic Detergent Sodium Dodecyl Sulfate. Indian J Microbiol 54, 134–138 (2014). https://doi.org/10.1007/s12088-013-0426-8

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  • DOI: https://doi.org/10.1007/s12088-013-0426-8

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