Abstract
In commercial tea production, plenty of tea leaf waste is generated, which may not only exert pollution risk to environment, but also a huge waste of bioactive ingredients in tea. In this study, the 4th to 7th leaves of tea bush were collected and used as substrate for mycelial culture of two renown medicinal mushrooms Grifola frondosa and Tianzhi (new variants of Ganoderma lucidum) to obtain a new type of solid-state fermented tea. Result showed that the polysaccharides of Grifola frondosa and Tianzhi fermented tea were 1.52 and 4.14 %, tea polyphenols were 1.51 and 1.85 %, the free amino acids were 1.52 and 0.94 %, caffeine were 1.16 and 1.70 %, polyphenols/amid acids ratio were 1.0 and 1.98, water extractions were 35.53 and 32.86 %, protein contents were 17.63 and 6.13 mg/g, respectively. The volatile components were mainly composed of alcohols, esters, aldehydes and ketones. The contents of major flavor compositions of fermented tea had changed and their relation tended to be harmonious, and the variety of amino acids significantly increased. Therefore, the sensory flavor and therapeutic qualities of fermented tea were significantly improved.
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References
Illana-Esteban CEl hongo (2008) maitake, (Grifola frondosa) y su potencial terape′utico. Rev Iberoam Micol 25:141–144
Lee BC, Bae JT, Pyo HB, Choe B, Kim SW, Wang HJ (2003) Biological activities of the polysaccharides produced from submerged culture of the edible Basidiomycete Grifola frondosa. Enzym Microbial Technol 32:574–581
Yang BK, Gu YA, Song CH (2007) Chemical characteristics and immunomodulating activities of exo-biopolymers produced by Grifola frondosa during submerged fermentation process. Int J Biol Macromol 41:227–233
Pegler DN (2002) Useful fungi of the world: the Ling-zhi–the mushroom of immortality. Mycologist 16:100–101
Liu G-Q, Zhang K-C (2005) Mechanisms of the anticancer action of Ganoderma lucidum (Leyss. ex. Fr.) Karst.: a new understanding. J Integr Plant Biol 47(2):129–135
Paterson RRM (2006) Ganoderma—a therapeutic fungal biofactory. Phytochemistry 67:1985–2001
Xie JT, Wang CZ, Wicks S, Yin JJ, Kong J, Li J et al (2006) Ganoderma lucidum extract inhibits proliferation of SW 480 human colorectal cancer cells. Exper Oncol 28:25–29
Cabrera C, Giménez R, López MC (2003) Determination of tea components with antioxidant activity. J Agric Food Chem 51(15):4427–4435
Lambert JD, Yang CS (2003) Cancer chemo preventive activity and bioavailability of tea and tea polyphenols. Mutat Res 523:201–208
Wu AH, Butler LM (2011) Green tea and breast cancer. Mol Nutr Food Res 55:921–930
Yang CS, Wang H, Li GX, Yang ZH, Guan F, Jin HY (2011) Cancer prevention by tea: evidence from laboratory studies. Pharmacol Res 64:113–122
Friedman M, Levin CE, Choi SH, Lee SU, Kozukue N (2009) Changes in the composition of raw tea leaves from the Korean yabukida plant during high temperature processing to pan-fried kamairi-cha green tea. J Food Sci 74:C406–C412
Molan AL, Sivakumaran S, Spencer PA, Meagher LP (2004) Green tea flavan-3-ols and oligomeric proanthocyanidins inhibit the motility of infective larvae of Teladorsagia circumcincta and Trichostrongylus colubriformis in vitro. Res Vet Sci 77:239–243
Kondo M, Kita K, Yokota H-o (2004) Effects of tea leaf waste of green tea, oolong tea, and black tea addition on sudangrass silage quality and in vitro gas production. J Sci Food Agric 84:721–727
Xi XG, Wei XL, Wang YF, Chu QJ, Xiao JB (2010) Determination of tea polysaccharides in camellia by a method phenol-sulfuric acid method. Arch Biol Sci 62(3):671–678
Bradford M (1976) A rapid and sensitive method for the quantification of microgram quantities of proteins using the principle of protein-dye-binding. Anal Biochem 72:248–254
Molan AL, Flanagan J, Wei W, Moughan PJ (2009) Selenium-containing green tea has higher antioxidant and prebiotic activities than regular green tea. Food Chem 114:829–835
Oleshko GI, Yarygina TI, Zorina EV, Reshetnikova MD (2011) Development of a unified procedure to measure the sum of free amino acids in raw medicinal plant materials and extraction preparations. Farmatsiya (Moscow) 3:14–17
El-Shahawi MS, Hamza A, Bahaffi SO, Al-Sibaai AA, Abduljabbar TN (2012) Analysis of some selected catechins and caffeine in green tea by high performance liquid chromatography. Food Chem 134(4):2268–2275
Thorngate JH, Noble AC (1995) Sensory evaluation of bitterness and astringency of 3R(-)-epicatechin and 3S(+)-catechin. Sci Food Agric 67:531–535
Arnáiz JE, Bernal MT, Martín MJ, Nozal JL, Bernal L (2012) Toribio supercritical fluid extraction of free amino acids from broccoli leaves. J Chromatogr A 1250:49–53
Jumtee K, Komura H, Bamba T, Fukusaki E (2011) Predication of Japanese green tea (Sen-cha) ranking by volatile profiling using gas chromatography mass spectrometry and multivariate analysis. J Biosci Bioeng 112(3):252–255
EGiles G, Mahoney CR, Brunyé TT, Gardony AL, Taylor HA, Kanarek RB (2012) Differential cognitive effects of energy drink ingredients: caffeine, taurine, and glucose. Pharmacol Biochem Behav 102(4):569–577
Yoto A, Murao S, Motoki M, Yokoyama Y, Horie N, Takeshima K, Masuda K, Kim M, Yokogoshi H (2012) Oral intake of c-aminobutyric acid affects mood and activities of central nervous system during stressed condition induced by mental tasks. Amino Acids 43(3):1331–1337
Harris RC, Wise JA, Price KA, Kim HJ, Kim CK, Sale C (2012) Determinants of muscle carnosine content. Amino Acids 43(1):5–12
Liang Y, Zhang L, Lu J (2005) A study on chemical estimation of Pu-erh tea quality. J Sci Food Agric 85(3):381–390
Gu X, Zhang Z, Wan X et al (2009) Simultaneous distillation extraction of some volatile flavor components from Pu-erh tea samples—comparison with steam distillation-liquid/liquid extraction and soxhlet extraction. Int J Anal Chem 10:1–6
Jumtee K, Komura H, Bamba T et al (2011) Predication of Japanese green tea (Sen-cha) ranking by volatile profiling using gas chromatography mass spectrometry and multivariate analysis. J Biosci Bioeng 112(3):252–255
Acknowledgments
We would like to thank the National Natural Science Foundation of China (Grant No. 30871768, 31272217), the Projects of Science & Technology of Guangdong Province (Grant Nos. 2011B090400412, 2012B020316004) for financial support.
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Bai, WF., Guo, XY., Ma, LQ. et al. Chemical Composition and Sensory Evaluation of Fermented Tea with Medicinal Mushrooms. Indian J Microbiol 53, 70–76 (2013). https://doi.org/10.1007/s12088-012-0345-0
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DOI: https://doi.org/10.1007/s12088-012-0345-0