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Streptomyces antibioticalis, a Novel Species from a Sanitary Landfill Soil

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Abstract

A novel isolate belonging to the genus Streptomyces, strain SL-4T, was isolated from soil sample collected from a sanitary landfill, New Delhi, India. The taxonomic status of this isolate was studied by polyphasic approach including morphological, physiological and chemo-taxonomic characterization. Spore chains of SL-4T were open loops, hooks or extended spirals of wide diameter (retinaculiperti). The cell wall peptidoglycan of the isolate SL-4T contained L,L-diaminopimelic acid, suggesting that the strain has a cell wall of chemotype-I. The polar lipid profile of the isolate was of Type II, with phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. The 16SrRNA gene sequence similarity between SL-4T and its phylogenetic relatives Streptomyces atrovirens NRRLB 16357 T (DQ026672), S. albogriseolus NRRLB 1305 T (AJ494865), S viridodiastaticus NBRC 13106 T (AB184317), S. caelestis NRRL 2418 T (X80824), S. flavoviridis NBRC 12772 T (AB184842), S. pilosus NBRC 12807 T (AB184161) and S. longispororuber NBRC 13488 T (AB184440) was 99.65, 99.65, 99.64, 99.23, 99.15, 99.14 and 99.13 % respectively. Subsequent DNA–DNA hybridization experiments with the test strain and its clade members showed 55.27, 44.27, 36.86, and 15.65 % relatedness between SL-4T and its relatives S. atrovirens, S. albogriseolus, S. viridodiastaticus and S. longispororuber respectively. The genotypic and phenotypic data was analyzed to verify possibility of the isolate SL-4T representing novel member of the genus Streptomyces, for which the name S. antibioticalis is being proposed. The type strain is SL-4T (=CCM 7434T=MTCC 8588T).

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Acknowledgments

This work was supported by grants from Ministry of Environment and Forests (MOEF), Government of India. RS acknowledges CSIR (Council of Scientific and Industrial Research), Government of India, for providing the Senior research fellowship. Infrastructural facilities provided by Acharya Narendra Dev College are gratefully acknowledged. We would like to thank J. P. Euzèby for etymological advice.

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  1. Acharya Narendra Dev College, University of Delhi, Govindpuri, Kalkaji, New Delhi, 110 019, India

    Monisha Khanna & Renu Solanki

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  1. Monisha Khanna
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  2. Renu Solanki
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Correspondence to Monisha Khanna.

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Khanna, M., Solanki, R. Streptomyces antibioticalis, a Novel Species from a Sanitary Landfill Soil. Indian J Microbiol 52, 605–611 (2012). https://doi.org/10.1007/s12088-012-0309-4

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