Skip to main content
SpringerLink
Log in

Production of Chitinase and its Optimization from a Novel Isolate Serratia marcescens XJ-01

  • Original Article
  • Published:
Indian Journal of Microbiology Aims and scope Submit manuscript

Abstract

Production of chitinase from bacteria has distinct advantages over fungi, due to the formation of mycelia of fungi in the later phase of fermentation. A novel chitinase-producing bacterial strain XJ-01 was isolated from the Yulu fishing field of Changsha, Hunan province, China, by enrichment and spread-plate technique, sequentially. Physicochemical characterization and 16S rRNA sequencing revealed that strain XJ-01 belongs to Serratia marcescens. By optimizing the fermentation condition based on L9(34) orthogonal experimental design, a maximal chitinase activity up to 15.36 U/ml was attained by that stain under the condition: 0.5% (NH4)2SO4 as the nitrogen source, 0.75% colloidal chitin as the carbon source, temperature of 32°C, time of 32 h and pH 8.0.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Deshpande MV (1986) Enzymatic degradation of chitin and its biological applications. J Sci Ind Res 45:277–281

    Google Scholar 

  2. Nicol S (1991) Life after death for empty shells. New Sci 129:46–48

    CAS  Google Scholar 

  3. Harish Prashanth KV, Tharanathan RN (2007) Chitin/chitosan: modifications and their unlimited application potential—an overview. Trends Food Sci Technol 18(3):117

    Article  Google Scholar 

  4. Gooday G (1990) The ecology of chitin decomposition. Adv Microb Ecol 11:387–430

    CAS  Google Scholar 

  5. Hoster F, Schmitz JE, Daniel R (2005) Enrichment of chitinolytic microorganisms: isolation and characterization of a chitinase exhibiting antifungal activity against phytopathogenic fungi from a novel Streptomyces strain. Appl Microbiol Biotechnol 66(4):434–442

    Article  PubMed  CAS  Google Scholar 

  6. Xiao X, Zhou Y, Wang F (2003) Isolation and identification of a high-efficient chitin-degrading marine bacterium CB101 and studies on its chitinases system [J]. Acta Oceanol Sin 1(1):138–142

    Google Scholar 

  7. Min T-l, Yang Q-y, Nang X-x, Zhou Y-m (2006) Isolation and identification of a Serratia marcescens from photosynthetic bacteria. J Anhui Agric Sci 34(22):5837–5838

    Google Scholar 

  8. Jin H, Ge X-r, Tao Y, Ran H-y, Liu S-g (2005) Identification of a pathogenic strain of locus and its toxicity and pathology. Acta Microbiol Sin 45(2):172–176

    Google Scholar 

  9. Xia J-l, Peng A-a, He H, Yang Y, Liu X-d, Qiu G-z (2007) A new strain Acidithiobacillus albertensis BY-05 for bioleaching of metal sulfides ores. Trans Nonferrous Met Soc China 17(1):168

    Article  CAS  Google Scholar 

  10. Hayashi K, Ito M (2002) Antidiabetic action of low molecular weight chitosan in genetically obese diabetic KK-A y mice. Biol Pharm Bull 25(2):188–192

    Article  PubMed  CAS  Google Scholar 

  11. Brurberg MB, Synstad B, Klemsdal SS, van Aalten DMF, Sundheim L, Eijsink VGH (2001) Chitinases from Serratia marcescens. Recent Res Dev Microbiol 5:187–204

    CAS  Google Scholar 

  12. Khanafari A, Assadi MM, Fakhr FA (2006) Review of prodigiosin, pigmentation in Serratia marcescens. Online Biol Sci 6(1):1–13

    Article  Google Scholar 

  13. Reid JD, Ogrydziak DM (1981) Chitinase-overproducing mutant of Serratia marcescens. Appl Environ Microbiol 41(3):664

    PubMed  CAS  Google Scholar 

  14. Xia J-l, Meng H, Wang R-m, Zhang C-g, Xiong J, Nie Z-y, Qiu G-z (2009) Identification and fermentation optimization of protopectinase-overproducing strain Aspergillus niger CD-01 for pectin production. J Cent South Univ Technol 16:0053–0060

    Article  Google Scholar 

  15. Green AT, Healy MG, Healy A (2005) Production of chitinolytic enzymes by Serratia marcescens QMB1466 using various chitinous substrates. J Chem Technol Biotechnol 80(1):28–34

    Article  CAS  Google Scholar 

  16. Kadokura K, Rokutani A, Yamamoto M, Ikegami T, Sugita H, Itoi S, Hakamata W, Oku T, Nishio T (2007) Purification and characterization of Vibrio parahaemolyticus extracellular chitinase and chitin oligosaccharide deacetylase involved in the production of heterodisaccharide from chitin. Appl Microbiol Biotechnol 75(2):357–365

    Article  PubMed  CAS  Google Scholar 

  17. Pan Z-q, Huang H-q, Shan Q, Bao S-x (2008) Optimization of fermentation conditions for chitinase production by Serratia sp. PS-2. Ind Microbiol 38(002):47–51

    CAS  Google Scholar 

  18. Chanpen W, Patcharaporn S, Amarat B (1999) Purification and characterization of chitinase from Bacillus circulans no. 4.1. Curr Microbiol 39:134–140

    Article  Google Scholar 

  19. Yu C-y, Han B-q, Li J, Liu W-s, Dai J-x (2002) The production condition and purification of chitinase from Vibrio pacini. High Technol Lett 9:70–73

    Google Scholar 

Download references

Acknowledgment

This work was supported by the National Natural Science Foundation of China (Nos. 50621063, 50674101).

Author information

Authors and Affiliations

  1. School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China

    Jin-Lan Xia, Jing Xiong, Rui-Yong Zhang, Ke-Ke Liu, Bin Huang & Zhen-Yuan Nie

  2. Key Laboratory of Biometallurgy of the Ministry of the Education of China, Central South University, Changsha, 410083, China

    Jin-Lan Xia & Zhen-Yuan Nie

Authors
  1. Jin-Lan Xia
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jing Xiong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rui-Yong Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ke-Ke Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Bin Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Zhen-Yuan Nie
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jin-Lan Xia.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xia, JL., Xiong, J., Zhang, RY. et al. Production of Chitinase and its Optimization from a Novel Isolate Serratia marcescens XJ-01. Indian J Microbiol 51, 301–306 (2011). https://doi.org/10.1007/s12088-011-0139-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12088-011-0139-9

Keywords

Search

Navigation