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Optimization of Antimicrobial Production by a Marine Actinomycete Streptomyces afghaniensis VPTS3-1 Isolated from Palk Strait, East Coast of India

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Abstract

Totally 25 marine soil samples were collected from the region of Palk Strait of Bay of Bengal, Tamil Nadu, and were subjected to the isolation of actinomycetes. Sixty-eight morphologically distinct isolates were obtained and 37% (25) of them had antimicrobial activity. The potential producer was named as Streptomyces sp. VPTS3-1 and the phylogenetic evaluation on the basis of 16S rDNA sequence further categorized the organism as Streptomyces afghaniensis VPTS3-1. Further, the antimicrobial compound was extracted from the isolate using various solvents and the antimicrobial efficacies were tested against bacterial and fungal pathogens. In addition, in vitro optimization of parameters for the antimicrobial compound production revealed that the suitable pH as 7–8, the period of incubation as 9 days, temperature (30°C), salinity (2%), and starch and KNO3 as the suitable carbon and nitrogen sources respectively in starch–casein medium.

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References

  1. Lazzarini A, Caveletti L, Toppo G, Marinelli F (2000) Rare genera of actinomycetes as potential of new antibiotics. Antonie van Leuwenhoek 78:399–405

    Article  CAS  Google Scholar 

  2. Saisivam S, Kishan V (2006) Taxonomy, fermentation and biological activities of a new strain of Streptomyces luridus from Indian soil. Ind J Microbiol 46:153–160

    Google Scholar 

  3. Wu XC, Chen WF, Qian CD, Li O, Li P, Wen YP (2007) Isolation and identification of newly isolated antagonistic Streptomyces sp. strain AP19-2 producing chromomycins. J Microbiol 45:499–504

    PubMed  CAS  Google Scholar 

  4. Lam KS (2006) Discovery of novel metabolites from marine actinomycetes. Curr Opin Microbiol 9:245–251

    Article  PubMed  CAS  Google Scholar 

  5. Imada C, Koseki N, Kamata M, Kobayashi T, Hamada-Sato N (2007) Isolation and characterization of antimicrobial substances produced by marine actinomycetes in the presence of seawater. Actinomycetologica 21:27–31

    Article  CAS  Google Scholar 

  6. Venkateswarlu G, Murali PS, Sharma G, Venkateswarlu R (2000) Improvement of rifamycin B production using mutant strains of Amycolatopsis mediterranei. Bioprocess Eng 23:315–318

    Article  CAS  Google Scholar 

  7. Gandhimathi R, Arunkumar M, Selvin J, Thangavelu T, Sivaramakrishnan S, Kiran GS, Shanmughapriya S, Natarajaseenivasan K (2008) Antimicrobial potential of sponge associated marine actinomycetes. J Med Mycol 18:16–22

    Google Scholar 

  8. Dhanasekaran D, Thajuddin N, Panneerselvam A (2008) An antifungal compound: 4′phenyl-1-napthyl–phenyl acetamide from Streptomyces sp. DPTB16. Facta Universitatis Ser Med Biol 15:7–12

    Google Scholar 

  9. Harindran J, Gupte TE, Naik SR (1999) HA-1-92, a new antifungal antibiotic produced by Streptomyces CDRIL-312: fermentation, isolation, purification and biological activity. World J Microbiol Biotechnol 15:425–430

    Article  CAS  Google Scholar 

  10. Fukuda DS, Mynderse JS, Baker PJ, Berry DM, Boeck LD, Yao RC, Mertz FP, Nakatsukasa WM, Mabe J, Ott J, Counter FT, Ensminger PW, Allen NE, Alborn W, Hobbs JN (1990) A80915, a new antibiotic complex produced by Streptomyces aculeolatus. Discovery, taxonomy, fermentation, isolation, characterization and antibacterial evaluation. J Antibiot 43:623–633

    Article  PubMed  CAS  Google Scholar 

  11. Ivanova V, Schlegel R (1997) Structure elucidation of the antibiotic demalonylniphimycin by two-dimensional NMR techniques. Actinomycetes 8:1–9

    Google Scholar 

  12. Vijayakumar R, Muthukumar C, Thajuddin N, Panneerselvam A, Saravanamuthu R (2007) Studies on the diversity of actinomycetes in the Palk Strait region of Bay of Bengal, India. Actinomycetologica 21:59–65

    Article  CAS  Google Scholar 

  13. Lu Z, Wang L, Zhang Y, Shi Y, Liu Z, Quintana ET, Goodfellow M (2003) Actinomadura catellatispora sp. nov. and Actinomadura glauciflava sp. nov., from a sewage ditch and soil in southern China. Int J Syst Evol Microbiol 53:137–142

    Article  PubMed  CAS  Google Scholar 

  14. Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:312–340

    Article  Google Scholar 

  15. Lechevalier MP, Lechevalier HA (1970) Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443

    Article  CAS  Google Scholar 

  16. Wilson K (1990) Preparation of genomic DNA from bacteria. In: Ausubel Kingstan RE, Moore DD, Smith JA, Seidman JG, Struhl K (eds) Current protocol in molecular biology, Chaps. 2–4. Greene Publishing and Wiley Inter Science, New York

    Google Scholar 

  17. Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S rDNA amplification for phylogenetic study. J Bacteriol 173:697–703

    PubMed  CAS  Google Scholar 

  18. Higgins DG, Bleasby AT, Fuchs R (1992) Clustal W: improved software for multiple sequence alignment. CABIOS 8:189–191

    PubMed  CAS  Google Scholar 

  19. Kimura MA (1980) Simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  PubMed  CAS  Google Scholar 

  20. Saitou N, Nei M (1987) The neighbour joining method: a new method for reconstructing phylogenetic trees from evolutionary distance data. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  21. Bordoloi GN, Kumarim B, Guha A, Bordoloi M, Yadav RN, Roy MK, Bora TC (2001) Isolation and structure elucidation of a new antifungal and antibacterial antibiotic produced by Streptomyces sp. 201. Biosci Biotechnol Biochem 65:1856–1858

    Article  PubMed  CAS  Google Scholar 

  22. Narayana KJP, Ravikiran D, Vijayalakshmi M (2005) Screening of Streptomyces species from cultivated soil for broad-spectrum antimicrobial compounds. Asian J Microbiol Biotechnol Environ Sci 7:121–124

    CAS  Google Scholar 

  23. Remya M, Vijayakumar R (2008) Isolation and characterization of marine antagonistic actinomycetes from West Coast of India. Facta Universitatis Ser Med Biol 15:13–19

    Google Scholar 

  24. Sahin N, Ugur A (2003) Investigation of the antimicrobial activity of some isolates. Turk J Biol 27:79–84

    Google Scholar 

  25. Augustine SK, Bhavsar SP, Baserisallhi M, Kapadnis BP (2004) Isolation, characterization and optimization of antifungal activity of actinomycetes of soil origin. Ind J Exp Biol 42:928–932

    CAS  Google Scholar 

  26. Kokare CR, Mahadik KR, Kadam SS, Chopade BA (2004) Isolation, characterization and antimicrobial activity of marine halophilic Actinopolyspora species AH1 from West Coast of India. Curr Sci 86:593–597

    Google Scholar 

  27. Kathiresan K, Balagurunathan R, Masilamani Selvam M (2005) Fungicidal activity of marine actinomycetes against phytopathogenic fungi. Ind J Biotechnol 4:271–276

    Google Scholar 

  28. De K, Gupta MK, Pande A (1992) Effect of physiological and physical factors on antifungal activities of Streptomyces spp. J Ind Bot Soc 72:157–159

    Google Scholar 

  29. Vijayakumar R, Seethalakshmi V, Anitha S, Saravanamuthu R (2009) Isolation and characterization of antagonistic actinomycetes from Coimbatore soils, Tamilnadu, India. J Sci Trans Environ Technol 2:191–201

    Google Scholar 

  30. Gupte MD, Kulkarni PR (2002) A study of antifungal antibiotic production by Streptomyces chattanoogensis MTCC 3423 using full factorial design. Lett Appl Microbiol 35:22–26

    Article  PubMed  CAS  Google Scholar 

  31. Rogers TO, Birnbaum J (1974) Biosynthesis of fosfomycin by Streptomyces fradiae. Antimicrob Agents Chemother 5:121–132

    Article  PubMed  CAS  Google Scholar 

  32. Kokare CR, Kadam SS, Mahadik KR, Chopade BA (2007) Studies on bioemulsifier production from marine Streptomyces sp. S1. Ind J Biotechnol 6:78–84

    CAS  Google Scholar 

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Acknowledgment

The authors would like to thank the Director, Indian Institute of Technology, Chennai, India for providing spectral analysis.

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Authors and Affiliations

  1. Department of Microbiology, Bharathidasan University College (W), Orathanadu, 614 625, India

    R. Vijayakumar

  2. Department of Microbiology, Dr. G.R. Damodaran College of Science, Coimbatore, 641 014, India

    K. Panneerselvam

  3. Department of Botany and Microbiology, College of Sciences, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia

    C. Muthukumar

  4. Department of Microbiology, Bharathidasan University, Tiruchirappalli, 620 024, India

    N. Thajuddin

  5. Department of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi, 613 503, India

    A. Panneerselvam

  6. Department of Botany, A.V.C. College (Autonomous), Mannampandal, 609 305, India

    R. Saravanamuthu

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  1. R. Vijayakumar
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  2. K. Panneerselvam
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  3. C. Muthukumar
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  4. N. Thajuddin
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  5. A. Panneerselvam
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  6. R. Saravanamuthu
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Correspondence to R. Vijayakumar.

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Vijayakumar, R., Panneerselvam, K., Muthukumar, C. et al. Optimization of Antimicrobial Production by a Marine Actinomycete Streptomyces afghaniensis VPTS3-1 Isolated from Palk Strait, East Coast of India. Indian J Microbiol 52, 230–239 (2012). https://doi.org/10.1007/s12088-011-0138-x

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  • DOI: https://doi.org/10.1007/s12088-011-0138-x

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