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Genetic Diversity and Pathogenicity of Xanthomonas axonopodis Strains Inducing Citrus Canker Disease in Iran and South Korea

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Abstract

For the first time in 1989 citrus bacterial canker disease has seen on Citrus aurantiifolia in southern Iran. A total of 43 strains from affected citrus trees, ten strains from South Korea and representative from all known five pathotypes of Xanthomonas axonopodis pathogenic on citrus trees were used in this study. Isolated strains from Iran were indistinguishable by phenotypic, FAMEs, and SDS-PAGE analyses but showed different host range. First group were pathogenic on all tested citrus seedlings including C. aurantiifolia, C. limettioides, C. limon, C. jambhiri, Poncirus trifoliata X C. paradisi, C. aurantium, C. paradise, C. medica, P. trifoliate, C. grandis, C. sinensis, C. reticulate and C. sinensis X P. trifoliate. Pathogenicity of the second group were limited to C. aurantiifolia, C. limettioides, C. limon, C. jambhiri, P. trifoliata X C. paradis, and C. aurantium. Among the strains studied by AFLP fingerprinting six clusters were found. These clusters were: (1) strains of pathotype C; (2) strains of pathotypes B and D; (3) strains of pathotype A together with the main group of the Iranian strains; (4) strains isolated from Korea; (5) strains of pathotype E; and (6) seven strains from Iran which made a completely separate cluster. Strains from pathotypes B and D could not be differentiated by AFLP. The tested Iranian strains belongs to the two different groups and strains from Korea grouped as a subcluster from main cluster of Iranian strains belong to the pathotype A.

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Notes

  1. Inn-Shik Myung, Overseas Pests Division, Department of Crop protection, National Institute of Agriculture Science and Technology, RDA, Suwonn 441-707, Korea.

References

  1. Alizadeh A, Rahimian H (1989) Citrus canker in Kerman province. Iran J Plant Pathol 26:118

    Google Scholar 

  2. De Vos P, Hogers R, Bleeker M, Reijans M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    Article  PubMed  CAS  Google Scholar 

  3. Gabriel DW, Hunter JE, Kingsley MT, Miller J, Lazo G (1988) Clonal population structure of Xanthomonas campestris and genetic diversity among citrus canker strains. Mol Plant Microbe Interact 1:59–65

    Article  Google Scholar 

  4. Gabriel DW, Kingsley MT, Hunter JE, Gotwald T (1989) Reinstatement of Xanthomonas citri (ex Hasse) and xanthomonas phaseoli (ex Smith) to species and reclassification of all X. campestris pv. citri strains. Int J Syst Bacteriol 39:14–22

    Article  Google Scholar 

  5. Gonzalez C, Restrepo S, Tohme J, Verdier V (2002) Characterization of pathogenic and nonpathogenic strains of Xanthomonas axonopodis pv. manihotis by PCR-based DNA fingerprinting techniques. FEMS Microbiol Lett 215:23–31

    Article  PubMed  CAS  Google Scholar 

  6. Gotwald TR, Graham JH, Civerolo EL, Barrett HC, Hearn CJ (1993) Differential host range reaction of citrus bacterial spot determined by leaf mesophil susceptibility. Plant Dis 77:1004–1009

    Article  Google Scholar 

  7. Graham JH, Hartung JS, Chase AR (1990) Pathological, restriction fragment length polymorphism and fatty acid profile relationship between Xanthomonas campestris from citrus and noncitrus hosts. Phytopathology 80:826–836

    Google Scholar 

  8. Hartung JS, Civerolo EL (1987) Genomic fingerprinting of Xanthomonas campestris pv. citri strains from Asia, South America and Florida. Phytopathology 80:826–836

    Google Scholar 

  9. Hartung JS, Civerolo EL (1989) Restriction fragment length polymorphism distinguish Xanthomonas strains isolated from Florida citrus nurseries from X. c. pv. citri. Phytopathology 79:793–799

    Article  Google Scholar 

  10. Janssen P, Coopman R, Huys G, Swings J, Bleeker M, Vos P, Zabeau M, Kersters K (1996) Evaluation of the DNA fingerprinting method AFLP as a new tool in bacterial taxonomy. J Gen Microbiol 142:1881–1893

    CAS  Google Scholar 

  11. Janssen P, Maquelin K, Coopman R, Tjernberg I, Bouvet P, Kersters K, Dijkshoorn L (1997) Discrimination of Acinetobacter genomic species by AFLP fingerprinting. Int J Syst Bacteriol 46:1179–1187

    Article  Google Scholar 

  12. Khodakaramian G, Swings J, Van Eygen S, Stewaert C, Rahimian H (2000) Grouping of the strains of Xanthomonas axonopodis inducing citrus canker and spot diseases from Asia, America and Australia on the bases of protein electrophoretic pattern (SDS-PAGE) and phenotypic features (BIOLOG). Iran J Plant Pathol 36:165–178

    Google Scholar 

  13. Khodakaramian G, Swings J, Vancaneyt M, Van Eygen S (2000) Fatty acid profiles of the strains of bacteria inducing citrus canker and spot diseases by gas liquid chromatography. Iran J Plant Pathol 36:197–206

    Google Scholar 

  14. Kolliker R, Kraehenbuehl R, Boller B, Widmer F (2006) Genetic diversity and pathogenicity of the grass pathogen Xanthomonas translucens pv. graminis. Syst Appl Microbiol 29:109–119

    Article  PubMed  Google Scholar 

  15. Nesme X, Vaneechoutte M, Orso S, Hoste B, Swings J (1995) Diversity and geneticrelatedness within genera Xanthomonas and Stenotrophomonas using restriction endonuclease site differences of PCR-amplified 16s rRNA gene. Syst Appl Microbiol 18:127–135

    CAS  Google Scholar 

  16. Pitcher DG, Saunders NA, Owen R (1989) Rapid extraction of bacterial genomic DNA with guanidine thiocyanate. Lett Appl Microbiol 8:151–156

    Article  CAS  Google Scholar 

  17. Rademaker JL, Hoste B, Louws FJ, Kersters K, Swings J, Vauterin L, Vauterin P, de Bruijn FJ (2000) Comparison of AFLP and rep-PCR genomic fingerprinting with DNA–DNA homology studies: Xanthomonas as a model system. Int J Syst Evol Microbiol 2:665–677

    Google Scholar 

  18. Restrepo S, Velez CM, Duque MC, Verdier V (2004) Genetic structure and population dynamics of Xanthomonas axonopodis pv. manihotis in Colombia from 1995 to 1999. Appl Environ Microbiol 70:255–261

    Article  PubMed  CAS  Google Scholar 

  19. Schoulties CL, Civerolo EL, Miller JW, Stall RE, Krass C, Poe SR, Ducharme EP (1987) Citrus canker in Florida. Plant Dis 71:388–395

    Article  Google Scholar 

  20. Simoes TH, Gonçalves ER, Rosato YB, Mehta A (2007) Differentiation of Xanthomonas species by PCR-RFLP of rpfB and atpD genes. FEMS Microbiol Lett 271:33–39

    Article  PubMed  CAS  Google Scholar 

  21. Stall RE, Civerolo EL (1991) Research relating to the recent outbreak of citrus canker in Florida. Phytopathology 29:399–400

    Article  CAS  Google Scholar 

  22. Vauterin L, Swings J, Kersters K (1991) Grouping of Xanthomonas campestris pathovars by SDS-PAGE of proteins. J Gen Microbiol 137:1677–1687

    CAS  Google Scholar 

  23. Vauterin L, Yang P, Hoste B, Vancanneyt M, Civerolo EL, Swings J, Kersters K (1991) Differentiation of Xanthomonas campestris pv. citri strains by sodium dodecyl sulfate polyacrylamide gel electrophoresis of proteins, fatty acid analysis and DNA–DNA hybridization. Int J Syst Bacteriol 41:535–542

    Article  CAS  Google Scholar 

  24. Vauterin L, Hoste B, Kersters K, Swings J (1995) Reclassification of Xanthomonas. Int J Syst Bacteriol 46:472–489

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Plant Protection, College of Agriculture, Bu Ali Sina University, Hamedan, Iran

    G. Khodakaramian

  2. Laboratorium Voor Microbiology, K. L. Ledeganckstraat 35, 9000, Ghent, Belgium

    J. Swings

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  1. G. Khodakaramian
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  2. J. Swings
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Correspondence to G. Khodakaramian.

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Khodakaramian, G., Swings, J. Genetic Diversity and Pathogenicity of Xanthomonas axonopodis Strains Inducing Citrus Canker Disease in Iran and South Korea. Indian J Microbiol 51, 194–199 (2011). https://doi.org/10.1007/s12088-011-0103-8

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  • DOI: https://doi.org/10.1007/s12088-011-0103-8

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