Abstract
We could not reproduce the model described by Lieber et al for nonalcoholic steatohepatitis model in rats. In our trial the high fat liquid diet group of rats gained nearly 100 g or less weight compared to the mean weight gain stated in the original article. However, the fasting glucose level was statistically higher in this group as compared to the chow diet group. Some pathological abnormalities in the duodenum and jejunum samples were observed in the high fat liquid diet group. We do not know the exact reason for these changes. Overall, our study results arose some suspicions about the reproducibility of the model. Furthermore, to the best of our knowledge, no study using the proposed model has been published so far.
Similar content being viewed by others
References
Lieber CS, Leo MA, Mak KM, Xu Y, Cao Q, Ren C, et al. Model of nonalcoholic steatohepatitis. Am J Clin Nutr 2004;79:502–9
Hill JO, Fried SK, DiGirolamo M. Effects of a high-fat diet on energy intake and expenditure in rats. Life Sci 1983;33(2):141–9
Corbett SW, Stern JS, Keesey RE. Energy expenditure in rats with diet-induced obesity. Am J Clin Nutr 1986;44(2):173–80
Watarai T, Kobayashi M, Takata Y, Sasaoka T, Iwasaki M, Shigeta Y. Alteration of insulin-receptor kinase activity by high-fat feeding. Diabetes 1988;37(10):1397–404
Harris RB. Factors influencing energy intake of rats fed either a high-fat or a fat mimetic diet. Int J Obes Relat Metab Disord 1994;18(9):632–40
Maegawa H, Kobayashi M, Ishibashi O, Takata Y, Shigeta Y. Effect of diet change on insulin action: difference between muscles and adipocytes. Am J Physiol 1986;251:E616–23
Pedersen O, Kahn CR, Flier JS, Kahn BB. High fat feeding causes insulin resistance and a marked decrease in the expression of glucose transporters (Glut 4) in fat cells of rats. Endocrinology 1991;129(2):771–7
Harrold JA, Widdowson PS, Clapham JC, Williams G. Individual severity of dietary obesity in unselected Wistar rats: relationship with hyperphagia. Am J Physiol Endocrinol Metab 2000;279(2):E340–7
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Akın, H., Deniz, M., Tahan, V. et al. High-fat liquid “Lieber-DeCarli” diet for an animal model of nonalcoholic steatohepatitis: does it really work?. Hepatol Int 1, 449–450 (2007). https://doi.org/10.1007/s12072-007-9028-z
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12072-007-9028-z