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Dual modified antiphospho (Ser10)-acetyl (Lys14)-histone H3 predominantly mark the pericentromeric chromatin during mitosis in monokinetic plants

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Abstract

Epigenetic regulatory posttranslational histone modification marks not only function individually but also capable to act in combination as a unique pattern. A total of 16 plant species belonging to 11 genera of eight families (five dicots and three monocots) including land plants, epiphytes (orchids) and the holokinetic taxa (Drosera spp.) were analysed for chromosomal distribution of dual modified antiphospho (Ser10)-acetyl (K14)-histone H3 (H3S10phK14ac) to understand the combinatorial chromatin dynamics during mitotic cell division in plants. The anti-H3S10phK14ac evidently mark the pericentromeric chromatin on mitotic chromosomes of the plants excluding the holokinetic Drosera species, which revealed the immunolabelling of whole chromosomes all along the arms. The dual modified immunosignals were absent during early stages of mitosis, appeared intensively at metaphase and remained visible until late-anaphase/telophase however, labelled the whole chromosomes during meiotic metaphase I. Colocalization of anti-H3S10phK14ac with an onion’s CENH3 antibody on mitotic chromosomes of Allium revealed the chromosomal location of anti-H3S10phK14ac in the region between signals for CENH3 detection. Overall analysis suggests that the unique localization of combinatorial histone modification mark at pericentromeric chromatin might have attributed through ‘phospho-acetyl’ cross talk that ultimately facilitate the sister chromatid cohesion at pericentromeres following condensation events in mitotic chromosomes. Here, we propose that dual modified H3S10phK14ac histone may serve as an additional cytogenetic landmark to identify pericentromeric chromatin during mitosis in plants. The plausible role of histone cross talk and future perspectives of combinatorial histone modification marks in plant cytogenetics with special reference to chromatin dynamics have been discussed.

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Acknowledgements

We thank Japan Society for the Promotion of Science (JSPS), Japan, for providing postdoctoral fellowship and research grant (SKS, no. P13399/2013). Sincere thanks are also to Dr Go Suzuki and all members of Plant Molecular Genetics Laboratory and Osaka Kyoiku University, Osaka, Japan, for their constant encouragement and help.

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Authors and Affiliations

  1. Laboratory of Plant Molecular Genetics, Division of Natural Sciences, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka, 582-8582, Japan

    SANTOSH KUMAR SHARMA & YASUHIKO MUKAI

  2. Department of Rehabilitation Sciences, Kansai University of Welfare Sciences, Kashiwara, Osaka, 582-0026, Japan

    MAKI YAMAMOTO

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  1. SANTOSH KUMAR SHARMA
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  2. MAKI YAMAMOTO
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  3. YASUHIKO MUKAI
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Correspondence to SANTOSH KUMAR SHARMA.

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Corresponding editor: Umesh C. Lavania

SKS, MY and YM designed the experiments and analysed the results. SKS conducted the experiments and wrote the manuscript

[Sharma S. K., Yamamoto M. and Mukai Y. 2016 Dual modified antiphospho (Ser10)-acetyl (Lys14)-histone H3 predominantly mark the pericentromeric chromatin during mitosis in monokinetic plants. J. Genet. 95, xx–xx]

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SHARMA, S.K., YAMAMOTO, M. & MUKAI, Y. Dual modified antiphospho (Ser10)-acetyl (Lys14)-histone H3 predominantly mark the pericentromeric chromatin during mitosis in monokinetic plants. J Genet 95, 965–973 (2016). https://doi.org/10.1007/s12041-016-0723-1

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  • DOI: https://doi.org/10.1007/s12041-016-0723-1

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