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Distribution and linkage disequilibrium analysis of polymorphisms of GH1 gene in different populations of pigs associated with body size

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Abstract

Growth hormone (GH) has been considered as a candidate gene for growth and body size in pigs. In this study, polymorphisms of the GH1 gene were evaluated for associations with body size traits in 190 pig individuals. Seventeen single-nucleotide polymorphisms (SNPs) were identified in GH1 gene of the large pig breeds and miniature pig breeds using direct sequencing and genotyped by allele-specific PCR approach. Notably, six (g.237A>G, g.283T>C, g.309A>G, g.318A>G, g.540A>G and g.544A>G) of them were significantly associated with body size, of which three loci (g.283T>C, g.309A>G, g.318A>G) located in the signal-peptide coding region of GH1 gene compose a CGG haplotype for large pigs and TAA haplotype for miniature pigs (P< 0.001), two loci (g.540A>G and g.544A>G) located in the second intron of GH1 gene compose a GG haplotype for large pigs and AA haplotype for miniature pigs (P< 0.001). Our results demonstrate that these SNPs in GH1 gene are associated with the body size of pigs providing genetic basis for pig breeding with the improved economic benefits.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31101781, 31072102) and the Programs Foundation of Ministry of Education of China (20110061110081).

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Authors and Affiliations

  1. College of Animal Science, Jilin University, 5333 Xi’an Road, Changchun, Jilin, 130062, People’s Republic of China

    YUNYUN CHENG, SONGCAI LIU, DAN SU, CHAO LU, XIN ZHANG, QINGYAN WU, SIMING LI, HAOYU FU, HAO YU & LINLIN HAO

  2. Five-Star Animal Health Pharmaceutical Factory of Jilian Province, 5333 Xi’an Road, Changchun, Jilin, 130062, People’s Republic of China

    SONGCAI LIU

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  1. YUNYUN CHENG
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  2. SONGCAI LIU
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  3. DAN SU
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  4. CHAO LU
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  5. XIN ZHANG
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  6. QINGYAN WU
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  7. SIMING LI
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  8. HAOYU FU
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  9. HAO YU
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  10. LINLIN HAO
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Correspondence to LINLIN HAO.

Additional information

Cheng Y., Liu S., Su D., Lu C., Zhang X., Wu Q., Li S., Fu H., Yu H. and Hao L. 2016 Distribution and linkage disequilibrium analysis of polymorphisms of GH1 gene in different populations of pigs associated with body size. J. Genet. 95, xx–xx

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CHENG, Y., LIU, S., SU, D. et al. Distribution and linkage disequilibrium analysis of polymorphisms of GH1 gene in different populations of pigs associated with body size. J Genet 95, 79–87 (2016). https://doi.org/10.1007/s12041-015-0611-0

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  • DOI: https://doi.org/10.1007/s12041-015-0611-0

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