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Molecular characterization of zeta class glutathione S-transferases from Pinus brutia Ten.

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Abstract

Glutathione transferases (GSTs; EC 2.5.1.18) play important roles in stress tolerance and metabolic detoxification in plants. In higher plants, studies on GSTs have focussed largely on agricultural plants. There is restricted information about molecular characterization of GSTs in gymnosperms. To date, only tau class GST enzymes have been characterized from some pinus species. For the first time, the present study reports cloning and molecular characterization of two zeta class GST genes, namely PbGSTZ1 and PbGSTZ2 from Pinus brutia Ten., which is an economically important pine native to the eastern Mediterranean region and have to cope with several environmental stress conditions. The PbGSTZ1 gene was isolated from cDNA, whereas PbGSTZ2 was isolated from genomic DNA. Sequence analysis of PbGSTZ1 and PbGSTZ2 revealed the presence of an open reading frame of 226 amino acids with typical consensus sequences of the zeta class plant GSTs. Protein and secondary structure prediction analysis of two zeta class PbGSTZs have shared common features of other plant zeta class GSTs. Genomic clone, PbGSTZ2 gene, is unexpectedly intronless. Extensive sequence analysis of PbGSTZ2, with cDNA clone, PbGSTZ1, revealed 87% identity at nucleotide and 81% identity at amino acid levels with 41 amino acids differences suggesting that genomic PbGSTZ2 gene might be an allelic or a paralogue version of PbGSTZ1.

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Acknowledgements

Thanks to Prof. Heinz Rennenberg and Dr Stanislav Kopriva from Forest Botanic and Tree Physiology Institute, Albert-Ludwigs University, Freiburg, Germany, for providing the initial EST sequences and their help in Northern blot and glutathione assays. Dr Elif Oztetik was supported by Tubitak short term fellowship. This work was financially supported by University of Anadolu Reseach Project.

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Authors and Affiliations

  1. Department of Biology, Anadolu University, 26470, Eskisehir, Turkey

    E. OZTETIK

  2. Department of Biology, Balikesir University, 10145, Balikesir, Turkey

    F. KOCKAR

  3. Department of Biology, Aksaray University, 68100, Aksaray, Turkey

    M. ALPER

  4. Department of Biological Sciences, Middle East Technical University (ODTU), 06800, Ankara, Turkey

    M. ISCAN

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  1. E. OZTETIK
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  2. F. KOCKAR
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  3. M. ALPER
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  4. M. ISCAN
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Correspondence to M. ALPER.

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[Oztetik E., Kockar F., Alper M. and Iscan M. 2015 Molecular characterization of zeta class glutathione S-transferases from Pinus brutia Ten. J. Genet. 94, xx–xx]

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OZTETIK, E., KOCKAR, F., ALPER, M. et al. Molecular characterization of zeta class glutathione S-transferases from Pinus brutia Ten.. J Genet 94, 417–423 (2015). https://doi.org/10.1007/s12041-015-0538-5

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  • DOI: https://doi.org/10.1007/s12041-015-0538-5

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