Abstract
Adipose tissue-derived stem cells (ADSCs) are one population of adult stem cells that can self renew and differentiate into multiple lineages. Because of advantages in method and quantity of acquisition, ADSCs are gaining attention as an alternative source of bone marrow mesenchymal stem cells. In this study, we performed microRNA profiling of undifferentiated and of neurally-differentiated ADSCs to identify the responsible microRNAs in neurogenesis using this type of stem cell. MicroRNAs from four different donors were analysed by microarray. Compared to the undifferentiation control, we identified 39–101 microRNAs with more than two-fold higher expression and 3–9 microRNAs with two-fold lower expression. The identified microRNAs were further analysed in terms of gene ontology (GO) in relation with neurogenesis, based on their target mRNAs predicted by computational analysis. This study revealed the specific microRNAs involved in neurogenesis via microRNA microarray, and may provide the basic information for genetic induction of adult stem cell differentiation using microRNAs.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ashjian P. H., Elbarbary A. S., Edmonds B., DeUgarte D., Zhu M., Zuk P. A. et al. 2003 In vitro differentiation of human processed lipoaspirate cells into early neural progenitors. Plast. Reconstr. Surg. 111, 1922–1931.
Behm-Ansmant I., Rehwinkel J. and Izaurralde E. 2006 MicroRNAs silence gene expression by repressing protein expression and/or by promoting mRNA decay. Cold Spring Harb. Symp. Quant. Biol. 71, 523–530.
Borge O. J. and Evers K. 2003 Aspects on properties, use and ethical considerations of embryonic stem cells – A short review. Cytotechnology 41, 59–68.
Chamberlain G., Fox J., Ashton B. and Middleton J. 2007 Concise review: mesenchymal stem cells: their phenotype, differentiation capacity, immunological features, and potential for homing. Stem Cells 25, 2739–2749.
Clarke D. and Frisen J. 2001 Differentiation potential of adult stem cells. Curr. Opin. Genet. Dev. 11, 575–580.
Du T. and Zamore P. D. 2005 microPrimer: the biogenesis and function of microRNA. Development 132, 4645–4652.
Duisters R. F., Tijsen A. J., Schroen B., Leenders J. J., Lentink V., van der Made I. et al. 2009 miR-133 and miR-30 Regulate connective tissue growth factor. Implications for a role of MicroRNAs in myocardial matrix remodeling. Circ. Res. 104, 170–178.
Esau C., Kang X., Peralta E., Hanson E., Marcusson E. G., Ravichandran L. V. et al. 2004 MicroRNA-143 regulates adipocyte differentiation. J. Biol. Chem. 279, 52361–52365.
Foshay K. M. and Gallicano G. I. 2007 Small RNAs, big potential: the role of MicroRNAs in stem cell function. Curr. Stem Cell Res. Ther. 2, 264–271.
Gregory R. I., Chendrimada T. P. and Shiekhattar R. 2006 MicroRNA biogenesis: isolation and characterization of the microprocessor complex. Methods Mol. Biol. 342, 33–47.
Huang Q., Gumireddy K., Schrier M., le Sage C., Nagel R., Nair S. et al. 2008 The microRNAs miR-373 and miR-520c promote tumour invasion and metastasis. Nat. Cell Biol. 10, 202–210.
In ’t Anker P. S., Scherjon S. A., Kleijburg-van der Keur C., de Groot-Swings G. M., Claas F. H., Fibbe W. E. et al. 2004 Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta. Stem Cells 22, 1338–1345.
Joglekar M. V., Parekh V. S. and Hardikar A. A. 2007 New pancreas from old: microregulators of pancreas regeneration. Trends Endocrinol. Metab. 18, 393–400.
Krichevsky A. M., Sonntag K. C., Isacson O. and Kosik K. S. 2006 Specific microRNAs modulate embryonic stem cell-derived neurogenesis. Stem Cells 24, 857–864.
Lakshmipathy U. and Hart R. P. 2008 Concise review: MicroRNA expression in multipotent mesenchymal stromal cells. Stem Cells 26, 356–363.
Lopez-Romero P., Gonzalez M. A., Collejas S., Dopazo A. and Irizarny R. A. 2010 Processing of Agilent microRNA array data. BMC Res. Notes 3, 18.
Lu L. L., Liu Y. J., Yang S. G., Zhao Q. J., Wang X., Gong W. et al. 2006 Isolation and characterization of human umbilical cord mesenchymal stem cells with hematopoiesis-supportive function and other potentials. Haematologica 91, 1017–1026.
Mallory A. C. and Vaucheret H. 2004 MicroRNAs: something important between the genes. Curr. Opin. Plant Biol. 7, 120–125.
Mizuno H. 2003 Versatility of adipose tissue as a source of stem cells. J. Nippon. Med. Sch. 70, 428–431.
Musina R. A., Bekchanova E. S., Belyavskii A. V. and Sukhikh G. T. 2006 Differentiation potential of mesenchymal stem cells of different origin. Bull. Exp. Biol. Med. 141, 147–151.
Pansky A., Roitzheim B. and Tobiasch E. 2007 Differentiation potential of adult human mesenchymal stem cells. Clin. Lab. 53, 81–84.
Passier R. and Mummery C. 2003 Origin and use of embryonic and adult stem cells in differentiation and tissue repair. Cardiovasc. Res. 58, 324–335.
Prockop D. J., Sekiya I. and Colter D. C. 2001 Isolation and characterization of rapidly self-renewing stem cells from cultures of human marrow stromal cells. Cytotherapy 3, 393–396.
Raposio E., Guida C., Baldelli I., Benvenuto F., Curto M., Paleari L. et al. 2007 Characterization and induction of human pre-adipocytes. Toxicol. In Vitro 21, 330–334.
Roh J. K., Jung K. H. and Chu K. 2008 Adult stem cell transplantation in stroke: its limitations and prospects. Curr. Stem Cell Res. Ther. 3, 185–196.
Schaffler A. and Buchler C. 2007 Concise review: adipose tissue-derived stromal cells – basic and clinical implications for novel cell-based therapies. Stem Cells 25, 818–827.
Tang F., Hajkova P., O’Carroll D., Lee C., Tarakhovsky A., Lao K. and Surani M. A. 2008 MicroRNAs are tightly associated with RNA-induced gene silencing complexes in vivo. Biochem. Biophys. Res. Commun. 372, 24–29.
Tavazoie S. F., Alarcon C., Oskarsson T., Padua D., Wang Q., Bos P. D. et al. 2008 Endogenous human microRNAs that suppress breast cancer metastasis. Nature 451, 147–152.
Tzur G., Levy A., Meiri E., Barad O., Spector Y., Bentwich Z. et al. 2008 MicroRNA expression patterns and function in endodermal differentiation of human embryonic stem cells. PLoS ONE 3, e3726.
Wang S. H., Bian C. J. and Zhao C. H. 2008 Expression and function of microRNA in embryonic stem cell. Yi Chuan. 30, 1545–1549.
Wilfred B. R., Wang W. X. and Nelson P. T. 2007 Energizing miRNA research: a review of the role of miRNAs in lipid metabolism, with a prediction that miR-103/107 regulates human metabolic pathways. Mol. Genet. Metab. 91, 209–217.
Zeng Y., Yi R. and Cullen B. R. 2005 Recognition and cleavage of primary microRNA precursors by the nuclear processing enzyme Drosha. EMBO J 24, 138–148.
Zuk P. A., Zhu M., Mizuno H., Huang J., Futrell J. W., Katz A. J. et al. 2001 Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 7, 211–228.
Author information
Authors and Affiliations
Corresponding author
Additional information
[Cho J. A., Park H., Lim E. H. and Lee K. W. 2011 MicroRNA expression profiling in neurogenesis of adipose tissue-derived stem cells. J. Genet. 90, 81–93]
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
CHO, J.A., PARK, H., LIM, E.H. et al. MicroRNA expression profiling in neurogenesis of adipose tissue-derived stem cells. J Genet 90, 81–93 (2011). https://doi.org/10.1007/s12041-011-0041-6
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12041-011-0041-6