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Modulatory effect of Tim-3/Galectin-9 axis on T-cell-mediated immunity in pulmonary tuberculosis

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Abstract

Patients affected by pulmonary tuberculosis (PTB) manifest deficiencies in innate cellular immunity. The Tim-3/Galectin-9 axis is an important regulator of Th1 cell immunity, leading to Th1 cell apoptosis. Herein, this study aims to clarify the underlying roles of the Tim-3/Galectin-9 axis in T-cell immunity in PTB. Peripheral blood mononuclear cells (PBMCs) were extracted from subjects with and without PTB to examine the expression of CD4, CD8, CD25, and Tim-3 under the stimulation of Mycobacterium tuberculosis (MTB) and purified protein derivative (PPD). In addition, the expression of Tim-3 and Galectin-9 in PBMCs was determined. The Tim-3/Galectin-9 axis in the PBMCs was activated or blocked to detect the secreted levels of IFN-γ, TNF-α, IL-2, and IL-22. MTB stimulation increased the expression of CD4, CD8, CD25, Tim-3, and Galectin-9 in PBMCs. The blockade of Tim-3/Galectin-9 axis resulted in reduced secretion of IFN-γ, TNF-α, IL-2, and IL-22 from T-cells. Moreover, Tim-3+CD4+T, Tim-3+CD8+, and Tim-3+CD25+T cells exhibited a greater ability to inhibit the replication of MTB in macrophages. Taken conjointly, the blockade of Tim-3/Galectin-9 axis inhibits the secretion of inflammatory cytokines in T-cells to regulate the T-cell immunity in PTB.

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Acknowledgements

This study was supported by National Natural Science Foundation of China (No. 81670080). We appreciate the helpful comments from the reviewers of this paper.

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Authors and Affiliations

  1. Department of Respiratory, The First Hospital of Jilin University, Changchun, 130021, People’s Republic of China

    Jing Kang & Ming-Xian Li

  2. Jilin Medical University, Jilin, 132013, People’s Republic of China

    Jing Kang

  3. Department of Cardiology, Jilin Province FAW General Hospital, Changchun, 130011, People’s Republic of China

    Zhi-Feng Wei

  4. Department of Pediatric Rheumatology, Immunology and Allergy, the First Hospital of Jilin University, Changchun, 130021, People’s Republic of China

    Jing-Hua Wang

Authors
  1. Jing Kang
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  2. Zhi-Feng Wei
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  3. Ming-Xian Li
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  4. Jing-Hua Wang
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Corresponding author

Correspondence to Jing-Hua Wang.

Additional information

Communicated by Dipankar Nandi.

Corresponding editor: Dipankar Nandi

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Supplementary Figure 1

Flow cytometric data for sorting CD8+ T cells from healthy controls, LPTB patients and PTB patients. In the HC group, n = 20; in the LPTB group, n = 40; and in the active PTB group, n = 50 (EPS 10283 kb)

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Kang, J., Wei, ZF., Li, MX. et al. Modulatory effect of Tim-3/Galectin-9 axis on T-cell-mediated immunity in pulmonary tuberculosis. J Biosci 45, 60 (2020). https://doi.org/10.1007/s12038-020-0023-z

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  • DOI: https://doi.org/10.1007/s12038-020-0023-z

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