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Elucidation and genetic intervention of CO2 concentration mechanism in Chlamydomonas reinhardtii for increased plant primary productivity

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Abstract

The rising global population is forcing the need for adapting alternative sustainable technologies for enhanced crop productivity. The CO2 Concentration Mechanisms (CCMs) evolved in algae to counter the inefficient CO2 fixing enzyme, RuBisCo and slower diffusion of CO2 in water offers good scope for the above purpose. The CCMs are single-celled CO2 supply mechanisms that depend on multiple CO2/HCO −3 transporters and acclimation states and accumulate 100-fold more CO2 than low CO2 environments. Although some insights have been obtained regarding the CCMs of blue-green algae and green algae like Chlamydomonas reinhardtii, further progress needs to take place to understand the molecular and biochemical basis for intracellular transport of CO2. In this review, complete information pertaining to the core CCM is presented and discussed in light of the available literature. In addition to this, information on CO2/HCO −3 sensing, photo-acclimation in low CO2, liquid-like nature of pyrenoid, untapped potential of high CO2 responses and high CO2 requiring mutants, and prospects of engineering CCM components into higher plants are presented and discussed.

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Acknowledgements

We acknowledge the funding from CSIR (No. 38 (1429)/17-EMRII), and INSA, New Delhi, to KM. KM also acknowledges Dr M Raghuram, head of the Botany and Microbiology Department, ANU, for providing administrative support. We also thank the Department of Science and Technology, New Delhi, for the DST-FIST programme.

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Authors and Affiliations

  1. Department of Botany and Microbiology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh, 522510, India

    Kokkanti Mallikarjuna, Kelam Narendra & Ragireddypalem Ragalatha

  2. Indian Institute for Science Education and Research (IISER), Tirupati, Andhra Pradesh, India

    Basuthkar J Rao

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  1. Kokkanti Mallikarjuna
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  2. Kelam Narendra
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  3. Ragireddypalem Ragalatha
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  4. Basuthkar J Rao
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Correspondence to Kokkanti Mallikarjuna.

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This article is part of the Topical Collection: Genetic Intervention in Plants: Mechanisms and Benefits.

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Mallikarjuna, K., Narendra, K., Ragalatha, R. et al. Elucidation and genetic intervention of CO2 concentration mechanism in Chlamydomonas reinhardtii for increased plant primary productivity. J Biosci 45, 115 (2020). https://doi.org/10.1007/s12038-020-00080-z

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  • DOI: https://doi.org/10.1007/s12038-020-00080-z

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