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Investigation of axonal regeneration of Triturus ivanbureschi by using physiological and proteomic strategies

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Abstract

Peripheral nerve injuries are frequently observed and successful treatment depends mainly on the injury type, location of the damage, and the elapsed time prior to treatment. The regenerative capacity is limited only to the embryonic period in many mammalian tissues, but urodele amphibians do not lose this feature during adulthood. The main purpose of this study is to define the recovery period after serious sciatic nerve damage of a urodele amphibian, Triturus ivanbureschi. Experimental transection damage was performed on the sciatic nerves of T. ivanbureschi specimens. The recovery period of sciatic nerves were investigated by walking track analysis, electrophysiological recordings, and bottom-up proteomic strategies at different time points during a 35-day period. A total of 34 proteins were identified related to the nerve regeneration process. This study showed that the expression levels of certain proteins differ between distal and proximal nerve endings during the regeneration period. In distal nerve stumps, transport proteins, growth factors, signal, and regulatory molecules are highly expressed, whereas in proximal nerve stumps, neurite elongation proteins, and cytoskeletal proteins are highly expressed.

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Acknowledgements

This work was supported by The Scientific and Technological Research Council of Turkey (TÜBİTAK) [Grant Number 113Z124] and is also part of a doctorate thesis (Seçil Karahisar Turan, Hacettepe University, Institute of Science). We are grateful to Dr. Ali Demirsoy for his great, ad hoc counseling. We also thank Beycan Ayhan, Hatice Yıldızhan, Naşit İğci, N. Pınar Barkan and Selen Peker for technical expertise. This study was funded by The Scientific and Technological Research Council of Turkey (TÜBİTAK) [Grant Number 113Z124].

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, Hacettepe University, 06800, Ankara, Turkey

    Seçil Karahisar Turan & Mehmet Ali Onur

  2. Department of Biomedical Engineering, Faculty of Engineering, Ankara University, 06830, Ankara, Turkey

    Fatma Duygu Özel Demiralp

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  1. Seçil Karahisar Turan
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  2. Mehmet Ali Onur
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  3. Fatma Duygu Özel Demiralp
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Correspondence to Fatma Duygu Özel Demiralp.

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Ethical approval

All Triturus ivanbureschi specimens were collected from their natural habitats with the permission of the Republic of Turkey, Ministry of Forestry and Water Affairs, General Directorate of Nature Conservation and National Parks (protocol B.23.0.DMP.0.15.01-510.02-9165). All experimental procedure was approved by Hacettepe University Animal Research Ethics Committee (protocol B.30.2.HAC.0.05.06.00/17).

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Communicated by Aurnab Ghose.

Corresponding editor: Aurnab Ghose

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Karahisar Turan, S., Onur, M.A. & Özel Demiralp, F.D. Investigation of axonal regeneration of Triturus ivanbureschi by using physiological and proteomic strategies. J Biosci 44, 145 (2019). https://doi.org/10.1007/s12038-019-9950-y

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  • DOI: https://doi.org/10.1007/s12038-019-9950-y

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