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Candidate gene makers for Candidatus Liberibacter asiaticus for detecting citrus greening disease

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An Erratum to this article was published on 05 June 2013

Abstract

Citrus Huanglongbing (HLB) also known as citrus greening is one of the most devastating diseases of citrus worldwide. The disease is caused by Candidatus Liberibacter bacterium, vectored by the psyllid Diaphorina citri Kuwayama and Trioza erytreae Del Guercio. Citrus plants infected by the HLB bacterium may not show visible symptoms sometimes for years following infection. The aim of this study was to develop effective gene-specific primer pairs for polymerase chain reaction based method for quick screening of HLB disease. Thirty-two different gene-specific primer pairs, across the Ca. Liberibacter asiaticus genome, were successfully developed. The possibility of these primer pairs for cross-genome amplification across ‘Ca. Liberibacter africanus’ and ‘Ca. Liberibacter americanus’ were tested. The applicability of these primer pairs for detection and differentiation of Ca Liberibacter spp. is discussed.

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Acknowledgements

This research was supported by the Florida Citrus Production Research Advisory Council, Florida, USA. The authors also thank Dr. William O Dawson for permission to use his laboratory and greenhouse facilities.

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Authors and Affiliations

  1. University of Florida, IFAS, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL, 33850, USA

    Madhugiri Nageswara-Rao, Stephen M Garnsey & Siddarame Gowda

  2. Department of Plant Sciences, The University of Tennessee, 252 Ellington Plant Sciences, 2431 Joe Johnson Dr., Knoxville, TN, 37996, USA

    Madhugiri Nageswara-Rao

  3. US Sugar Corporation, Clewiston, FL, 33440, USA

    Mike Irey

Authors
  1. Madhugiri Nageswara-Rao
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  2. Mike Irey
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  3. Stephen M Garnsey
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  4. Siddarame Gowda
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Corresponding authors

Correspondence to Madhugiri Nageswara-Rao or Siddarame Gowda.

Additional information

Corresponding editor: Man Mohan Johri

MS received 14 August 2012; accepted 12 March 2013

Corresponding editor: Man Mohan Johri

[Nageswara-Rao M, Irey M, Garnsey SM and Gowda S 2013 Candidate gene makers for Candidatus Liberibacter asiaticus for detecting citrus greening disease. J. Biosci. 38 1–9] DOI 10.1007/s12038-013-9315-x

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Nageswara-Rao, M., Irey, M., Garnsey, S.M. et al. Candidate gene makers for Candidatus Liberibacter asiaticus for detecting citrus greening disease. J Biosci 38, 229–237 (2013). https://doi.org/10.1007/s12038-013-9315-x

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  • DOI: https://doi.org/10.1007/s12038-013-9315-x

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