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An insight into the sequential, structural and phylogenetic properties of banana 1-aminocyclopropane-1-carboxylate synthase 1 and study of its interaction with pyridoxal-5′-phosphate and aminoethoxyvinylglycine

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Abstract

In banana, ethylene production for ripening is accompanied by a dramatic increase in 1-aminocyclopropane-1-carboxylate (ACC) content, transcript level of Musa acuminata ACC synthase 1 (MA-ACS1) and the enzymatic activity of ACC synthase 1 at the onset of the climacteric period. MA-ACS1 catalyses the conversion of S-adenosyl-L-methionine (SAM) to ACC, the key regulatory step in ethylene biosynthesis. Multiple sequence alignments of 1- aminocyclopropane-1-carboxylate synthase (ACS) amino acid sequences based on database searches have indicated that MA-ACS1 is a highly conserved protein across the plant kingdom. This report describes an in silico analysis to provide the first important insightful information about the sequential, structural and phylogenetic characteristics of MA-ACS1. The three-dimensional structure of MA-ACS1, constructed based on homology modelling, in combination with the available data enabled a comparative mechanistic analysis of MA-ACS1 to explain the catalytic roles of the conserved and non-conserved active site residues. We have further demonstrated that, as in apple and tomato, banana- ACS1 (MA-ACS1) forms a homodimer and a complex with cofactor pyridoxal-5′-phosphate (PLP) and inhibitor aminoethoxyvinylglycine (AVG). We have also predicted that the residues from the PLP-binding pocket, essential for ligand binding, are mostly conserved across the MA-ACS1 structure and the competitive inhibitor AVG binds at a location adjacent to PLP.

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Abbreviations

AATase:

aspartate aminotransferase

ACC:

1-aminocyclopropane-1-carboxylate

AVG:

aminoethoxyvinylglycine

CDD:

Conserved Domain Database

DOPE:

discrete optimized protein energy

MA-ACS1 :

Musa acuminata ACC synthase 1

MACC:

N-manonyl ACC

MTA:

5′ methylthioadenosine

MVG:

methoxyvinylglycine

PLP:

pyridoxal-5′-phosphate

RMSD:

root mean square deviation

SAM:

S-adenosyl-L-methionine

TATase:

tyrosine aminotransferase

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Authors and Affiliations

  1. Department of Botany, Bose Institute, 93/1, APC Road, Kolkata, 700 009, India

    Swarup Roy Choudhury, Sanjay Kumar Singh & Dibyendu N. Sengupta

  2. Protein Chemistry Laboratory, Department of Chemistry, Bose Institute, 93/1, A.P.C Road, Kolkata, 700 009, India

    Sujit Roy

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  1. Swarup Roy Choudhury
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  2. Sanjay Kumar Singh
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  3. Sujit Roy
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  4. Dibyendu N. Sengupta
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Correspondence to Dibyendu N. Sengupta.

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These authors have contributed equally to this work.

Supplementary figures and table pertaining to this article are available on the Journal of Biosciences Website at http://www.ias.ac.in/jbiosci/June2010/pp281-294/suppl.pdf

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Choudhury, S.R., Singh, S.K., Roy, S. et al. An insight into the sequential, structural and phylogenetic properties of banana 1-aminocyclopropane-1-carboxylate synthase 1 and study of its interaction with pyridoxal-5′-phosphate and aminoethoxyvinylglycine. J Biosci 35, 281–294 (2010). https://doi.org/10.1007/s12038-010-0032-4

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