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Alcohol and Cocaine Exposure Modulates ABCB1 and ABCG2 Transporters in Male Alcohol-Preferring Rats

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Abstract

Two efflux transporters, ATP-binding cassettes B1 (ABCB1) and G2 (ABCG2), are highly expressed in the endothelial cells of the brain, where they regulate the bioavailability and distribution of several endogenous and xenobiotic compounds. However, whether ABCB1 or ABCG2 has any link with drug dependence, drug withdrawal effects, or the incidence of adverse effects in drug abuser is not known. In this study, we determined the effects of voluntary ethanol consumption following repeated exposure to cocaine or vehicle on the relative mRNA and protein expression of Abcg2/ABCG2 and Abcb1/ABCB1 in the nucleus accumbens (NAc) and medial prefrontal cortex (mPFC) of male alcohol-preferring (P) rats. Male P rats were allowed free choice access to ethanol (15 and 30% v/v) and water for 5 weeks to establish baseline drinking behavior. The following week, rats were either injected with 20 mg/kg i.p. of cocaine or saline, once a day, for 7 days. The relative mRNA and protein expression of Abcb1/ABCB1 and Abcg2/ABCG2 in the NAc and mPFC were significantly decreased in ethanol-saline- and ethanol-cocaine-exposed rats compared to control rats that received neither ethanol nor cocaine. Thus, prolonged exposure to commonly abused drugs, ethanol and cocaine, alters the expression of Abcb1/ABCB1 and Abcg2/ABCG2 mRNA and protein levels in brain areas that play a role in drug dependence.

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Acknowledgments

The authors thank Sujan Chandra Das and Yusuf S. Althobaiti for their contribution to the experimental procedures. We thank Dr. Charles R. Ashby Jr. (St. John’s University, Queens, NY) and Ms. Charisse Montgomery (University of Toledo, Toledo, Ohio) for critically reviewing the manuscript.

Funding

This work was supported, in part, by the National Institutes of Health (R01AA019458 to Y.S.) and the University of Toledo start-up grant (F110760) to A.K.T.

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  1. Alaa M. Hammad

    Present address: Department of Pharmacy, College of Pharmacy, Al-Zaytoonah University of Jordan, Amman, Jordan

  2. Alaa M. Hammad and Fawaz Alasmari contributed equally to this work.

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  1. Department of Pharmacology and Experimental Therapeutics, College of Pharmacy and Pharmaceutical Sciences, University of Toledo, Health Science Campus, 3000 Arlington Avenue, Toledo, OH, 43614, USA

    Alaa M. Hammad, Fawaz Alasmari, Youssef Sari, F. Scott Hall & Amit K. Tiwari

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Hammad, A.M., Alasmari, F., Sari, Y. et al. Alcohol and Cocaine Exposure Modulates ABCB1 and ABCG2 Transporters in Male Alcohol-Preferring Rats. Mol Neurobiol 56, 1921–1932 (2019). https://doi.org/10.1007/s12035-018-1153-2

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