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Tibolone Preserves Mitochondrial Functionality and Cell Morphology in Astrocytic Cells Treated with Palmitic Acid

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Abstract

Obesity has been associated with increased chronic neuroinflammation and augmented risk of neurodegeneration. This is worsened during the normal aging process when the levels of endogenous gonadal hormones are reduced. In this study, we have assessed the protective actions of tibolone, a synthetic steroid with estrogenic actions, on T98G human astrocytic cells exposed to palmitic acid, a saturated fatty acid used to mimic obesity in vitro. Tibolone improved cell survival, and preserved mitochondrial membrane potential in palmitic acid-treated astrocytic cells. Although we did not find significant actions of tibolone on free radical production, it modulated astrocytic morphology after treatment with palmitic acid. These data suggest that tibolone protects astrocytic cells by preserving both mitochondrial functionality and morphological complexity.

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Acknowledgments

YG-G is supported by a PhD fellowship from Centro de Estudios Interdisciplinarios Básicos y Aplicados CEIBA (Rodolfo Llinás Program). We acknowledge support from Ministerio de Economía y Competitividad (MINECO), Spain (grant no. BFU2014-51836-C2-1-R), CIBERFES, and Fondos FEDER.

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Authors and Affiliations

  1. Departamento de Nutrición y Bioquímica, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá D.C., Colombia

    Yeimy González-Giraldo & George E. Barreto

  2. Instituto Cajal, CSIC, Madrid, Spain

    Luis Miguel Garcia-Segura

  3. CIBER de Investigación Biomédica en Red de Fragilidad y Envejecimiento Saludable (CIBERFES), Instituto de Salud Carlos III, Madrid, Spain

    Luis Miguel Garcia-Segura

  4. Fac. Cs de la Salud, Universidad San Sebastián, Lientur 1457, 4080871, Concepción, Chile

    Valentina Echeverria

  5. Bay Pines VA Health Care System, 10,000 Bay Pines Blvd, Bldg 23, Rm 123, Bay Pines, FL, 33744, USA

    Valentina Echeverria

  6. Instituto de Ciencias Biomédicas, Universidad Autónoma de Chile, Santiago, Chile

    George E. Barreto

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  1. Yeimy González-Giraldo
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González-Giraldo, Y., Garcia-Segura, L.M., Echeverria, V. et al. Tibolone Preserves Mitochondrial Functionality and Cell Morphology in Astrocytic Cells Treated with Palmitic Acid. Mol Neurobiol 55, 4453–4462 (2018). https://doi.org/10.1007/s12035-017-0667-3

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