Abstract
The aim of this study was to evaluate the prognostic value of serum and cerebrospinal fluid (CSF) free fatty acid (FFA) levels in a cohort of patients with an acute ischemic stroke (AIS). In a prospective study, FFA levels were measured using an enzyme cycling method on admission in serum and CSF of 252 consecutive patients with AIS. The prognostic value of FFA to predict the functional outcome and mortality within 90-day was compared with the National Institutes of Health Stroke Scale score and with other known outcome predictors. Serum and CSF levels of FFA increased with increasing severity of stroke as defined by the NIHSS score (all P < 0.001). Patients with an unfavorable outcomes and non-survivors had significantly increased FFA serum and CSF levels on admission (all P < 0.0001). Multivariate logistic regression analysis adjusted for common risk factors showed that serum FFA ≥0.71 mmol/L (third quarters) was an independent predictor of functional outcome (odds ratios (OR) = 4.86; 95 % confidence interval (CI) 2.26–10.48) and mortality (OR = 7.72; 95 % CI 3.01–21.48). The area under the receiver operating characteristic curve of serum FFA was 0.79 (95 % CI, 0.72–0.86) for functional outcome and 0.86 (95 % CI, 0.78–0.94) for mortality. Similarly, CSF FFA level also was an indicator for predicting of functional outcome and mortality. FFA levels in serum and CSF may serve as independent biomarkers in addition of the traditional methods for assessing the functional outcome and mortality of AIS.
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Acknowledgments
This work was supported by the subject of Sichuan Province Health Department (150077), the subject of Luzhou Medical College (2013ZRQN082), and the subject of the affiliated hospital (14020). We also express our gratitude to all the patients who participated in this study and thereby made this work possible.
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On behalf of all authors, the corresponding author (Duan XX) states that there is no conflict of interest.
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This study was approved by the Institutional Review Board of the Affiliated Hospital of Luzhou Medical College.
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Duan, XX., Zhang, GP., Wang, XB. et al. Elevated Serum and Cerebrospinal Fluid Free Fatty Acid Levels Are Associated with Unfavorable Functional Outcome in Subjects with Acute Ischemic Stroke. Mol Neurobiol 54, 1677–1683 (2017). https://doi.org/10.1007/s12035-016-9756-y
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DOI: https://doi.org/10.1007/s12035-016-9756-y