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Enhancement of Neural Stem Cell Survival, Proliferation, Migration, and Differentiation in a Novel Self-Assembly Peptide Nanofibber Scaffold

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A Correction to this article was published on 06 June 2023

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Abstract

Considerable efforts have been made to combine biologically active molecules into the self-assembling peptide in order to improve cells growth, survival, and differentiation. In this study, a novel three-dimensional scaffold (RADA4GGSIKVAV; R-GSIK) was designed by adding glycine and serine between RADA4 and IKVAV to promote the strength of the peptide. The cell adhesion, viability, proliferation, migration, and differentiation of rat embryonic neural stem cells (NSCs) in R-GSIK were investigated and compared to laminin-coated, two-dimensional, and Puramatrix cultures. The scanning electron microscopy studies of the R-GSIK showed an open porous structure and a suitable surface area available for cell interaction. R-GSIK promoted the cell adhesion, viability, proliferation, and migration compared to the other cultures. In addition, the R-GSIK enhanced NSCs differentiation into neuronal cells. The NSCs injected in R-GSIK had a lower glial differentiation rate than in the Puramatrix. The results suggest that R-GSIK holds great promise for cell therapies and neuronal tissue repair.

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Acknowledgements

This work was supported by the Shefa Neuroscience Center, Tehran, Iran, grant related to Dr-Thesis 31528 and Iran National Science Foundation, Tehran, Iran (INSF).

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Authors and Affiliations

  1. Histology and Embryology Group, Basic Science Department, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran

    Sajad Sahab Negah & Zabihollah Khaksar

  2. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran

    Sajad Sahab Negah, Sayed Mostafa Modarres Mousavi, Ali Jahanbazi Jahan-Abad & Ali Gorji

  3. Department of Neuroscience, Mashhad University of Medical Sciences, Mashhad, Iran

    Sajad Sahab Negah & Ali Gorji

  4. Department of Neuroscience, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran

    Hadi Aligholi

  5. Department of Plastic and Reconstructive Surgery, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Shahin Mohammad Sadeghi

  6. Pediatric Department, Medical Faculty, Shahed University, Tehran, Iran

    Hadi Kazemi

  7. Department of Clinical Biochemistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Ali Jahanbazi Jahan-Abad

  8. Department of Neurology and Department of Neurosurgery, Westfälische Wilhelms-Universität Münster, Münster, Germany

    Ali Gorji

  9. Epilepsy Research Center, Westfälische Wilhelms-Universität Münster, Münster, Germany

    Ali Gorji

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  1. Sajad Sahab Negah
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  2. Zabihollah Khaksar
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  4. Shahin Mohammad Sadeghi
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  5. Sayed Mostafa Modarres Mousavi
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  6. Hadi Kazemi
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  7. Ali Jahanbazi Jahan-Abad
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  8. Ali Gorji
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Correspondence to Ali Gorji.

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The authors declare that they have no conflict of interest.

Additional information

Sajad Sahab Negah and Zabihollah Khaksar contributed equally to this work.

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Sahab Negah, S., Khaksar, Z., Aligholi, H. et al. Enhancement of Neural Stem Cell Survival, Proliferation, Migration, and Differentiation in a Novel Self-Assembly Peptide Nanofibber Scaffold. Mol Neurobiol 54, 8050–8062 (2017). https://doi.org/10.1007/s12035-016-0295-3

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  • DOI: https://doi.org/10.1007/s12035-016-0295-3

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