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Corilagin Protects Against HSV1 Encephalitis Through Inhibiting the TLR2 Signaling Pathways In Vivo and In Vitro

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Abstract

In this study, we tried to explore the molecular mechanism that Corilagin protected against herpes simplex virus-1 encephalitis through inhibiting the TLR2 signaling pathways in vivo and in vitro. As a result, Corilagin significantly prevented increase in the levels of TLR2 and its downstream mediators following Malp2 or HSV-1 challenge. On the other hand, in spite of TLR2 knockdown, Corilagin could still significantly suppress the expression of P38 and NEMO, phosphor-P38, and nuclear factor kappa B. The mRNA and protein expression of TLR2 and its downstream mediators in the brain tissue were also significantly lowered in mice treated with Corilagin. In addition, Corilagin inhibited expression of tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 protein. In conclusion, Corilagin shows the potential to protect against HSV-1-induced encephalitis, and the beneficial effects may be mediated by inhibiting TLR2 signaling pathways.

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Acknowledgments

This study is sponsored by China National Natural Science Fund No. 81100894, No. 81371840, and No. 30901248.

Disclosure of Potential Conflicts of Interest

No potential conflicts of interest were disclosed.

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Authors and Affiliations

  1. Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People’s Republic of China

    Yuan-Jin Guo, Tao Luo, Huan Liu, Yuan-Wu Mei & Yuan Fang

  2. Department of Neurology, Wuhan General Hospital of Guangzhou Military Command, Wuhan, 430070, People’s Republic of China

    Fei Wu

  3. Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People’s Republic of China

    Hua-Rong Li, Shu-Ling Zhang & Lei Zhao

  4. School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, 430065, People’s Republic of China

    Jun-Yan Tao

  5. Central Lab, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People’s Republic of China

    Ji-Hua Dong

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  1. Yuan-Jin Guo
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  2. Tao Luo
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  3. Fei Wu
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  4. Huan Liu
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Correspondence to Lei Zhao.

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Yuan-Jin Guo and Tao Luo contributed equally to this study.

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Guo, YJ., Luo, T., Wu, F. et al. Corilagin Protects Against HSV1 Encephalitis Through Inhibiting the TLR2 Signaling Pathways In Vivo and In Vitro. Mol Neurobiol 52, 1547–1560 (2015). https://doi.org/10.1007/s12035-014-8947-7

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  • DOI: https://doi.org/10.1007/s12035-014-8947-7

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