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The Decrease on Na+, K+-ATPase Activity in the Cortex, but not in Hippocampus, is Reverted by Antioxidants in an Animal Model of Sepsis

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Abstract

In the present study, we investigated whether sepsis induced by cecal ligation and puncture (CLP) modifies Na+, K+-ATPase activity, mRNA expression, and cerebral edema in hippocampus and cerebral cortex of rats and if antioxidant (ATX) treatment prevented the alterations induced by sepsis. Rats were subjected to CLP and were divided into three groups: sham; CLP—rats were subjected to CLP without any further treatment; and ATX–CLP plus administration of N-acetylcysteine plus deferoxamine. Several times (6, 12, and 24) after CLP or sham operation, the rats were killed and hippocampus and cerebral cortex were isolated. Na+, K+-ATPase activity was inhibited in the hippocampus 24 h after sepsis, and ATX treatment was not able to prevent this inhibition. The Na+, K+-ATPase activity also was inhibited in cerebral cortex 6, 12, and 24 h after sepsis. No differences on Na+, K+-ATPase catalytic subunit mRNA levels were found in the hippocampus and cerebral cortex after sepsis. ATX treatment prevents Na+, K+-ATPase inhibition only in the cerebral cortex. Na+, K+-ATPase inhibition was not associated to increase brain water content. In conclusion, the present study demonstrated that sepsis induced by CLP inhibits Na+, K+-ATPase activity in a mechanism dependent on oxidative stress, but this is not associated to increase brain water content.

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Acknowledgments

This research was supported by grants from Programa de Pós-graduação em Ciências da Saúde—UNESC and Conselho Nacional de Desenvolvimento Científico e Tecnológico.

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Authors and Affiliations

  1. Laboratório de Fisiopatologia Experimental, Programa de Pós-graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil

    Isabela Casagrande Jeremias, Giselli Scaini, Larissa Constantino, Francieli Vuolo, Felipe Dal-Pizzol & Emilio Luiz Streck

  2. Laboratório de Neuroproteção e Doenças Metabólicas, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

    Andreia Kurek Ferreira, Emilene Barros Silva Scherer, Janaina Kolling & Angela Terezinha de Souza Wyse

  3. Laboratório de Biologia Genômica e Molecular, Departamento de Biologia Celular e Molecular, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil

    Arethuza da Silva Dornelles & Maurício Reis Bogo

  4. Instituto Nacional de Ciência e Tecnologia Translacional em Medicina (INCT-TM), Porto Alegre, RS, Brazil

    Maurício Reis Bogo, Felipe Dal-Pizzol & Emilio Luiz Streck

  5. Laboratório de Fisiopatologia Experimental, Universidade do Extremo Sul Catarinense, Av. Universitária, 1105, Criciúma, 88806-000, SC, Brazil

    Emilio Luiz Streck

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  1. Isabela Casagrande Jeremias
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  2. Giselli Scaini
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  3. Larissa Constantino
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  4. Francieli Vuolo
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  5. Andreia Kurek Ferreira
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  6. Emilene Barros Silva Scherer
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  7. Janaina Kolling
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  8. Arethuza da Silva Dornelles
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  9. Angela Terezinha de Souza Wyse
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  10. Maurício Reis Bogo
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  11. Felipe Dal-Pizzol
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  12. Emilio Luiz Streck
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Corresponding author

Correspondence to Emilio Luiz Streck.

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Isabela Casagrande Jeremias and Giselli Scaini contribute equally to this work.

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Jeremias, I.C., Scaini, G., Constantino, L. et al. The Decrease on Na+, K+-ATPase Activity in the Cortex, but not in Hippocampus, is Reverted by Antioxidants in an Animal Model of Sepsis. Mol Neurobiol 46, 467–474 (2012). https://doi.org/10.1007/s12035-012-8297-2

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  • DOI: https://doi.org/10.1007/s12035-012-8297-2

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