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Hsa-miR-199a-5p Protect Cell Injury in Hypoxia Induces Myocardial Cells Via Targeting HIF1α

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Abstract

Myocardial infarction (MI) is one of the most common global diseases. Recently, microRNA 199a-5p (miR-199a-5p) has been recognized as a vital regulator in several human diseases. Nevertheless, the function of miR-199a-5p and the associated downstream molecular mechanisms in myocardial injury remain undescribed. Here, we assessed the relative expression of miR-199a-5p in an oxidative stress injury model of human myocardial cells. The effects of miR-199a-5p on myocardial cell viability were determined by cell counting kit-8 (CCK-8), terminal deoxynucleotidyl transferase UTP nick end labeling (TUNEL), flow cytometry, and western blot assays. Online bioinformatic analysis was used to predict the aim of miR-199a-5p in cardiomyocyte injury, which was confirmed by dual-luciferase reporter assays. miR-199a-5p increased the growth rate of cardiomyocytes after treatment with a hypoxic environment. miR-199a-5p acted as an inhibitor directly targeted hypoxia-inducible factor-1 (HIF1α) expression, which was higher in the cardiomyocyte injury model than that in healthy myocardial cells. Upregulated HIF1α expression abolished miR-199a-5p-induced cell proliferation in the cardiomyocyte hypoxia model. Our results suggest that miR-199a-5p is a potential prognostic biomarker in myocardial damage.

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Acknowledgements

This study was supported by grants from the Science and Technology Project of Guangzhou City (201804010067) and Science and Technology Planning Project of Guangdong Province (2017ZC0064).

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Authors and Affiliations

  1. Department of Cardiothoracic Surgery, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, People’s Republic of China

    Hui-Yong Chen, Jun Lu, Zheng-Kang Wang, Jie Yang, Xiao Ling, Peng Zhu & Shao-Yi Zheng

  2. Department of Thoracic Surgery, Yuebei People’s Hospital, Shantou University, Shaoguan, People’s Republic of China

    Hui-Yong Chen

  3. Department of Cardiothoracic Surgery, Nanfang hospital, Southern Medical University, 1838 Guangzhou Avenue North, Baiyun, Guangzhou, Guangdong, 510280, People’s Republic of China

    Peng Zhu & Shao-Yi Zheng

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  1. Hui-Yong Chen
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Chen, HY., Lu, J., Wang, ZK. et al. Hsa-miR-199a-5p Protect Cell Injury in Hypoxia Induces Myocardial Cells Via Targeting HIF1α. Mol Biotechnol 64, 482–492 (2022). https://doi.org/10.1007/s12033-021-00423-7

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  • DOI: https://doi.org/10.1007/s12033-021-00423-7

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