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Functional Characterization of a Novel Glycosyltransferase (UGT73CD1) from Iris tectorum Maxim. for the Substrate promiscuity

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Abstract

Glycosylflavonoids are a class of natural products with multiple pharmacological activities and a lot of glycosyltransferases from various plant species have been reported that they were involved in the biosynthesis of these phytochemicals. However, no corresponding glycosyltransferase has been identified from the famous horticultural and medicinal plant Iris tectorum Maxim. Here, UGT73CD1, a novel glycosyltransferase, was identified from I. tectorum. based on transcriptome analysis and functional identification. Phylogenetic analysis revealed that UGT73CD1 grouped into the clade of flavonoid 7-OH OGTs. Biochemical analysis showed that UGT73CD1 was able to glycosylate tectorigenin at 7-OH to produce tectoridin, and thus assigned as a 7-O-glycosyltransferase. In addition, it also possessed robust catalytic promiscuity toward 12 structurally diverse flavonoid scaffolds and 3, 4-dichloroaniline, resulting in forming O- and N-glycosides. This work will provide insights into efficient biosynthesis of structurally diverse flavonoid glycosides for drug discovery.

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Funding

This study was supported by Guangdong Key Laboratory for translational Cancer research of Chinese Medicine (No. 2018B030322011), the National Natural Science Foundation of China (CN) (No.81874333 and 82003895) and the Guangdong Basic and Applied Basic Research Foundation (No.2020A1515010926).

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Authors and Affiliations

  1. Joint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education of the People’s Republic of China, International Institute for Translational Chinese Medicine, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, People’s Republic of China

    Jia Huang, Jing Li, Jingyang Yue, Zilu Huang, Libing Zhang, Wei Yao, Rui Guan, Jianlian Wu, Jincai Liang, Lixin Duan & Aijia Ji

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  1. Jia Huang
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Huang, J., Li, J., Yue, J. et al. Functional Characterization of a Novel Glycosyltransferase (UGT73CD1) from Iris tectorum Maxim. for the Substrate promiscuity. Mol Biotechnol 63, 1030–1039 (2021). https://doi.org/10.1007/s12033-021-00364-1

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