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Investigation of the effect of rhamnetin on mice injected with solid and ehrlich ascites tumor

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Abstract

Rhamnetin is a flavonoid which contained in especially clove, such as apple, tea, and onion plant. Rhamnetin has been used in cancer research due to its antitumor and antioxidant properties. In this study, effects of rhamnetin administration at different doses on ascites and solid tumors were investigated in Balb/C mice bearing EAT model that originating from rat breast adenocarcinoma. Experimental procedure: Overall, 92 Balb-c mice were used in this study. EAT cells (1 × 106 cells) that harvested from stock animals were injected to all rats via intraperitoneal and subcutaneous route. Rhamnetin (100 µg/kg–200 µg/kg) were given intraperitoneally and subcutaneously during 10 and 15 days to the animals bearing ascites tumor and solid tumor, respectively. Throughout experiments, weight changes were recorded in all groups. The maximum weight increase was observed in the control group among all groups (ascites and solid tumor groups). In the treatment groups, the least weight increase were determined in 200-µg/kg rhamnetin applied. The lowest increase in tumor volume was observed in the group that received 200-µg/kg rhamnetin (2.84) when compared to tumor control group (3.67). Result and conclusion: We determined that the number of live and dead cells in the treatment groups administered with the mean rhamnetin dose (2.5 µg/ml) was found in the count made in the EAT cell line after the incubation periods. We observed that rhamnetin plays an important role against cancer formation. We have obtained important results in our study, but detailed studies on the relationship between rhamnetin and cancer are needed.

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Data availability

Data sharing not applicable to this article as no datasets were generated or analysed during the current study.

Abbreviations

µg:

Microgram

µl:

Microliter

cm:

Centimeter

kg:

Kilogram

g:

Gram

mg:

Milligram

h:

Hour

DAB:

Diaminobenzidine

DAPI:

4,6-Diamidino-2-phenylindole

TdT:

Terminal deoxynucleotidyl transferase

dUTP:

Nonisotopic labeled nucleotides

TUNEL:

Terminal deoxynucleotidyl transferase-mediated dUTP Nick-end Labeling

EAT:

Ehrlich Ascites Tumor

PS:

Physiological saline

FITCH:

Fluorescein isothiocyanate

FVIII:

Factor VIII

ml:

Milliliter

mm3 :

Cubic millimeter

NSCLC:

Non-small cell lung cancer cells

PBS:

Phosphate buffer saline

DNA:

Deoxyribonucleic acid

W:

Watt

ip:

Intraperitoneal

sc:

Subcutaneous

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Acknowledgements

This research was produced from Özlem Bozkurt’s PhD thesis named “The investigating the antitumoral effect of rhamnetin on the mice,” in which Ehrlich ascites and solid tumor is created. Financial source of this research was funded by Erciyes University Scientific Research Projects Coordination Unit.

Funding

Bilimsel Araştırma Projeleri, Erciyes Üniversitesi, TDK-2014-5247, Erdoğan Unur

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Authors and Affiliations

  1. Faculty of Dentistry, Nevsehir Hacı Bektas Veli University, 2000 Evler Mah. Zübeyde Hanım Cad. 50300, Nevsehir, Turkey

    Ö. Bozkurt, U. Kökbaş & I. Alkan

  2. Faculty of Medicine, Department of Anatomy, Yozgat Bozok University, Yozgat, Turkey

    S. Yılmaz

  3. Faculty of Medicine, Department of Anatomy, KTO Karatay University, Konya, Turkey

    Ş. Alpa

  4. Faculty of Medicine, Department of Anatomy, Erciyes University, Kayseri, Turkey

    M. Nisari, Ö. Al & E. Unur

  5. Faculty of Medicine, Histology-Embryology Department, Erciyes University, Kayseri, Turkey

    A. H. Yay

  6. Faculty of Medicine, Department of Anatomy, Afyonkarahisar Health Sciences University, Afyon, Turkey

    T. Ertekin

  7. Faculty of Medicine, Department of Anatomy, Ordu University, Ordu, Turkey

    A. Tokpınar

  8. Oral and Dental Health Program, Cappadocia University, Nevsehir, Turkey

    A. Bozkurt

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  1. Ö. Bozkurt
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Correspondence to Ö. Bozkurt.

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Bozkurt, Ö., Yılmaz, S., Alpa, Ş. et al. Investigation of the effect of rhamnetin on mice injected with solid and ehrlich ascites tumor. Med Oncol 40, 124 (2023). https://doi.org/10.1007/s12032-023-01981-3

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