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Borealin/Dasra B is overexpressed in colorectal cancers and contributes to proliferation of cancer cells

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Abstract

Borealin/Dasra B, also called cell division cycle associated 8, is a member of the chromosomal passenger complex and plays a crucial role during mitosis and cell division. The expression of this gene has been investigated in several kinds of cancers, but its role in colorectal cancers (CRC) remains to be elucidated. In this study, our in silico analysis using the Oncomine database showed that Borealin was overexpressed in CRC tissues, which was further validated by immunohistochemistry in a cohort of 81 pairs of primary CRC and adjacent non-cancerous tissues. In CRC, Borealin expression was positively correlated with age (P = 0.0079), lymph node metastasis (P = 0.0086), and TNM stage (P = 0.0050). Significant difference in the overall survival time was seen between patients with high and low expression (P < 0.0001). Furthermore, high Borealin expression remained independently prognostic in multivariate analysis (HR 4.556, 95 % CI 1.964–10.567, P = 0.0004). Functional studies showed that suppression of Borealin expression with siRNA could significantly inhibit the growth and induce apoptosis in HCT-116 cells (P < 0.01). These results suggest that Borealin might act as an independent prognostic factor and a potential therapeutic target for patients with CRC.

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Acknowledgments

This work was supported by the Medical Talented Youth Project of Hongkou District, National Science Foundation of China (81273282, 81202353, 30972730), 973 Foundation, Shanghai Municipal Commission for Science and Technology (11JC1410902), and Changhai Hospital (CH125530300).

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Authors and Affiliations

  1. Department of General Surgery, Jiangwan Hospital, Shanghai, 200434, China

    Yong Wang, Zheng Zhao, Xiaoping Bao, Yong Fang, Ping Ni & Qiang Chen

  2. Department of Colorectal Surgery, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China

    Wei Zhang

  3. Department of Laboratory Diagnostic, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China

    Anmei Deng

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  1. Yong Wang
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  2. Zheng Zhao
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  3. Xiaoping Bao
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  4. Yong Fang
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  5. Ping Ni
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  6. Qiang Chen
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  7. Wei Zhang
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  8. Anmei Deng
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Corresponding authors

Correspondence to Wei Zhang or Anmei Deng.

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Yong Wang and Zheng Zhao have contributed equally to this work.

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Wang, Y., Zhao, Z., Bao, X. et al. Borealin/Dasra B is overexpressed in colorectal cancers and contributes to proliferation of cancer cells. Med Oncol 31, 248 (2014). https://doi.org/10.1007/s12032-014-0248-5

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  • DOI: https://doi.org/10.1007/s12032-014-0248-5

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