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Immunohistochemical analysis revealed CD34 and Ki67 protein expression as significant prognostic factors in colorectal cancer

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Abstract

CD34, cytokeratin (CK) 19, cytokeratin (CK) 20, and Ki67 have been demonstrated to be involved in tumor invasion and angiogenesis. The aim of this study was to analyze the clinicopathological significance of CD34, CK19, CK20, and Ki67 expressions in colorectal cancer (CRC) and to evaluate their involvement in the progression of CRC. CD34, CK19, CK20, and Ki67 expressions were assessed in paraffin-embedded specimens collected from 152 cases of CRC and 30 paired normal colorectal tissues by immunohistochemistry. The relationships between CD34, CK19, CK20, and Ki67 expressions and CRC were evaluated. The association of CD34 and Ki67 protein expressions with the clinicopathological characteristics and the prognosis of CRC were subsequently assessed. CD34, CK19, CK20, and Ki67 expressed highly in CRC tissues relative to normal colorectal tissues. Using immunostaining scoring, a significant correlation of CD34 and Ki67 with the UICC staging and histo-differentiation of CRC was found (P < 0.05), but no such correlation of CK19 and CK20 with the UICC staging and histo-differentiation (P > 0.05). Meanwhile, no relationship of CD34, CK19, CK20, and Ki67 with the location of CRC was found (P > 0.05). Patients with high expressions of CD34 and Ki67 had the lowest survival (P < 0.05). The results suggest that concurrent expression of CD34 and Ki67 may be an important characteristic of CRC which may help in the prediction of CRC progression.

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Acknowledgments

This study was supported by the financial grants from the Shanghai Science and Technology Development Fund (No. 05DJ14010), the Major Basic Research Program of Shanghai (No. 07DZ19505), and the National 973 Basic Research Program of China (No. 2008CB517403).

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  1. Department of Surgery, The Sixth People’s Hospital Affiliated to Shanghai Jiaotong University, 600 Yishan Road, Shanghai, 200233, China

    Yan-Lei Ma, Jia-Yuan Peng, Peng Zhang, Wei-Jie Liu, Long Huang & Huan-Long Qin

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  1. Yan-Lei Ma
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  2. Jia-Yuan Peng
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Correspondence to Huan-Long Qin.

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Ma, YL., Peng, JY., Zhang, P. et al. Immunohistochemical analysis revealed CD34 and Ki67 protein expression as significant prognostic factors in colorectal cancer. Med Oncol 27, 304–309 (2010). https://doi.org/10.1007/s12032-009-9210-3

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  • DOI: https://doi.org/10.1007/s12032-009-9210-3

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