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Identification and Characterization of Human MIBP1 Gene in Glioma Cell Differentiation

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Abstract

Malignant gliomas are the most common and lethal intracranial tumors; differentiation therapy is a promising candidate for their treatment. In order to reveal the mechanisms related to glioma differentiation, after confirming that differentiation was induced by sodium phenylbutyrate in SHG-44 human glioma cells, RNA arbitrary primer differential display was used to screen differentially expressed genes. One gene was found to be upregulated by differential display, and this was also confirmed by reverse northern blot and quantitative real-time PCR analysis. After it was cloned and sequenced, the 505-bp fragment was identified as the MIBP1 (c-myc intron-binding protein 1) gene, also named Hivep2/MBP-2/Schnurri-2. Quantitative real-time PCR analysis of 30 human tissue samples revealed that the expression of MIBP1 tended to decrease with increasing WHO grade and was significantly depressed in the high malignancy gliomas group (WHO grade IV). We cloned and sequenced the MIBP1 gene, which was accepted by GenBank as number DQ231041. Finally, transfection of MIBP1 in a reverse transcription vector into glioma cells inhibited cell growth, induced differentiation, and blocked the cell cycle. Here, we identify and describe the structure and function of a differentiation-related gene, human MIBP1, in human glioma.

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Acknowledgments

This work was supported by grants from the Tianjin Municipal Science and Technology Commission (Grant number 033800911 and 12ZCDZSY17700).

Conflict of Interest

We declare that we have no conflict of interest.

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Authors and Affiliations

  1. Department of Neurosurgery, Tianjin Huanhu Hospital, 122 Qixiangtai Road, Tianjin, 300060, People’s Republic of China

    Lijun Sun & Dashi Zhi

  2. Laboratory of Molecular Genetics, College of Life Sciences, Nankai University, 94 Weijin Road, Tianjin, 300071, People’s Republic of China

    Xiwen Chen, Weilan Wang & Defu Chen

  3. Tianjin Children’s Hospital, 225 Machang Street, Tianjin, 300074, People’s Republic of China

    Xingnan Jin

  4. Department of Neurosurgery, Second Affiliated Hospital, Suzhou University, 1055 Sanxiang Road, Suzhou, Jiangsu, 215004, People’s Republic of China

    Lijun Sun & Qiang Huang

Authors
  1. Lijun Sun
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  2. Xiwen Chen
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  3. Xingnan Jin
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  4. Qiang Huang
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  5. Weilan Wang
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  6. Dashi Zhi
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  7. Defu Chen
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Correspondence to Lijun Sun or Defu Chen.

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Sun, L., Chen, X., Jin, X. et al. Identification and Characterization of Human MIBP1 Gene in Glioma Cell Differentiation. J Mol Neurosci 52, 294–301 (2014). https://doi.org/10.1007/s12031-013-0144-z

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  • DOI: https://doi.org/10.1007/s12031-013-0144-z

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