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Immunohistochemical Characteristics and Distribution of Sensory Dorsal Root Ganglia Neurons Supplying the Urinary Bladder in the Male Pig

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Abstract

The study determined the distribution and immunohistochemical coding of the sensory neurons innervating the male pig urinary bladder. Retrograde tracer Fast Blue was injected bilaterally into the bladder trigone, base or dome. The presence of neuropeptide Y (NPY), somatostatin (SOM), galanin (GAL), vasoactive intestinal polypeptide (VIP), nitric oxide synthase (NOS), calcitonin gene-related peptide (CGRP) and substance P (SP) were studied with immunofluorescence. Fast Blue-positive neurons were localized bilaterally in dorsal root ganglia from L1 to L6 and from S3 to S4 with specific differences regarding the injection site. The number of Fast Blue-positive neurons was higher in the right ganglia. Immunohistochemistry revealed that sensory neurons innervating the urinary bladder trigone, base and dome displayed immunoreactivities to CGRP, SP, NOS, GAL and SOM. Differences in the neuropeptide content were observed between the Fast Blue-positive neurons in lumbar and sacral ganglia. Taken together, these data indicate that the lumbar and sacral pathways probably play different roles in sensory transmission from the urinary bladder trigone, base and dome.

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Acknowledgments

The author thanks M. Marczak and A. Penkowski for their excellent technical assistance. This study was supported by a grant NN 308 2334 38 from the National Committee for Scientific Research Poland.

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  1. Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego Street 13, 10-719, Olsztyn, Poland

    Zenon Pidsudko

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Correspondence to Zenon Pidsudko.

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Pidsudko, Z. Immunohistochemical Characteristics and Distribution of Sensory Dorsal Root Ganglia Neurons Supplying the Urinary Bladder in the Male Pig. J Mol Neurosci 52, 71–81 (2014). https://doi.org/10.1007/s12031-013-0117-2

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  • DOI: https://doi.org/10.1007/s12031-013-0117-2

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