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Cyclooxygenase-2 Genetic Polymorphism and Stroke Subtypes in Chinese

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Abstract

Background

Cyclooxygenase-2 (COX-2) catalyzes the formation of prostaglandins that contribute to the inflammation in atherosclerosis. The aim of the present study was to investigate the relationship between two polymorphisms (−1195G>A and -765G>C) in the COX-2 gene and subtypes of ischemic stroke in a Chinese population.

Methods

Genomic DNA of 224 patients with large artery atherosclerosis (LAA), 329 patients with small vessel occlusion (SVO), and 450 controls were genotyped for the COX-2 1195G>A (rs689466) and -765G>C (rs20417) polymorphisms using polymerase chain reaction–restriction fragment length polymorphism. Chi-square test and logistic regression analysis were performed for association analysis.

Results

The frequencies of variant allele with -1195G>A and -765G>C polymorphisms were 0.46 and 0.22, respectively. The -1195GA genotype and 1195A allele carriers were identified independently to be related with ischemic stroke (adjusted OR = 1.51, 95 % CI: 1.09–2.10, P = 0.02; OR = 1.45, 95 % CI: 1.06–1.97, P = 0.02) and SVO (adjusted OR = 1.57, 95 % CI: 1.07–2.30, P = 0.02; OR = 1.50, 95 % CI: 1.05–2.16, P = 0.03). In contrast, the 1195G>A polymorphism was not associated with LAA. No relationship between the -765G>C polymorphism and risk of either ischemic stroke was observed. The linkage disequilibrium analysis showed that -1195G>A and -765G>C SNPs are moderate linkage disequilibrium in this study population (D′ = 0.72, r 2 = 0.16). Compared with G-1195-G-765 haplotype, the haplotype of A-1195-G-765 showed significant increased risk of ischemic stroke (OR = 1.27, 95 % CI: 1.05–1.54, P = 0.02) and SVO (OR = 1.27, 95 % CI: 1.02–1.58, P = 0.03) but not LAA.

Conclusions

In conclusion, we found that -1195G>A polymorphism and A-1195-G-765 haplotype of COX-2 were associated with susceptibility to ischemic stroke in a Chinese population. The effects were confined to SVO among the stroke subtypes rather than to LAA.

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Source of funding

This study was supported by a grant of the Jinhua Science and Technology Bureau project, Zhejiang Province, the people's republic of china (No: 2008-3-055).

Conflicts of interest

The authors declare that there is no conflict of interest.

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Authors and Affiliations

  1. Tongde Hospital of Zhejiang Province, Hangzhou, 311122, Zhejiang Province, People’s Republic of China

    Guo-zhong Chen

  2. Department of Clinical Laboratory, Jinhua Municipal Central Hospital, Jinhua, 321000, Zhejiang Province, People’s Republic of China

    Xiao-yun Shan

  3. Department of Neurology, Jinhua People’s Hospital, Jinhua, 321000, Zhejiang Province, People’s Republic of China

    Gan-ping Cheng

  4. School of Medicine, Jinhua Polytechnic, No. 1188, Wuzhou Street, Jinhua City, 321017, Zhejiang Province, People’s Republic of China

    Hong-miao Tao

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  1. Guo-zhong Chen
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  2. Xiao-yun Shan
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Correspondence to Hong-miao Tao.

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Chen, Gz., Shan, Xy., Cheng, Gp. et al. Cyclooxygenase-2 Genetic Polymorphism and Stroke Subtypes in Chinese. J Mol Neurosci 51, 467–473 (2013). https://doi.org/10.1007/s12031-013-0078-5

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  • DOI: https://doi.org/10.1007/s12031-013-0078-5

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