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The Expression Changes of Cystathionine-β-synthase in Brain Cortex After Traumatic Brain Injury

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Abstract

Cystathionine-β-synthase (CBS) catalyzes the condensation of serine with homocysteine to form cystathionine and occupies a crucial regulatory position between the methionine cycle and the biosynthesis of cysteine by transsulfuration. It was reported that CBS was a novel marker of both differentiation and proliferation for certain cell types, suggesting that CBS represents a survival-promoting protein. However, its expression and function in the central nervous system lesion are not well understood. To investigate changes of CBS after traumatic brain injury (TBI) and its possible role, mice TBI model was established by controlled cortical impact system, and the expression and cellular localization of CBS after TBI was investigated in the present study. Western blot analysis revealed that CBS was present in normal mice brain cortex. It gradually decreased, reached a valley at the third day after TBI, and then restored to basal level. Importantly, more CBS was colocalized with neuron. In addition, Western blot detection showed that the third day postinjury was also the apoptosis peak indicated by the elevated expression of caspase-3. Importantly, immunohistochemistry analysis revealed that injury-induced expression of CBS was colabeled by Bcl-2 and had no co-localization with caspase-3. These data suggested that CBS may be implicated in the apoptosis of neuron and involved in the pathophysiology of brain after TBI.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 30872666, 81172911, and No. 81271379), Natural Science Foundation of Medical College of Nantong University (No. Y201003), The Priority Academic Program Development of Jiangsu Higher Education Institutions, and The Colleges and Universities in Jiangsu Province Plans to Graduate Research and Innovation (CXLX12_0824).

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Authors and Affiliations

  1. Institute of Forensic Sciences, Soochow University, Suzhou, 215123, Jiangsu, China

    Mingyang Zhang, Yaoqi Wang, Tao Wang, Weili Liu, Long Wang, Lu Zhang, Pan Chang, Wenwen Dong, Xiping Chen & Luyang Tao

  2. Department of Forensic Sciences, Medical College of Nantong University, Nantong, 226001, Jiangsu, China

    Mingyang Zhang

  3. Department of Obstetrics and Gynecology, North District of Suzhou Municipal Hospital, Suzhou, 215008, Jiangsu, China

    Haiyan Shan

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  1. Mingyang Zhang
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Correspondence to Xiping Chen or Luyang Tao.

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M. Zhang and H. Shan contributed equally to this work.

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Zhang, M., Shan, H., Wang, Y. et al. The Expression Changes of Cystathionine-β-synthase in Brain Cortex After Traumatic Brain Injury. J Mol Neurosci 51, 57–67 (2013). https://doi.org/10.1007/s12031-012-9948-5

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  • DOI: https://doi.org/10.1007/s12031-012-9948-5

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