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Temporal Distribution of Hig-1 (Hypoxia-Induced Gene 1) mRNA and Protein in Rat Spinal Cord: Changes During Postnatal Life

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Abstract

Several cellular and molecular events responsible for the development of the central nervous system (CNS), particularly those related to the development of ordered neural connections, occur during the first days of postnatal life, being days 1 through 10 a critical period to reach maturity and establish innervations. We have previously characterized hypoxia-induced gene 1 (Hig-1) and described an increase in its expression from day 1 to 15 of postnatal life in the spinal cord. Hig-1 mRNA has an open reading frame for a 93 amino acid protein, but its function has not been completely elucidated. Recently, several analyses in many cell types have related Hig-1 expression with differentiation or cell death/survival balance. With the aim of further characterizing the presence of Hig-1 in the CNS, we analyzed the cellular distribution of HIG-1 protein in rat's spinal cord at postnatal days 1, 8, 15, and 90 (P1–P90). We found an interesting change in the protein expression pattern, shifting from neurons at P1 to glial cells at P90, which points towards a functional role for this protein in the spinal cord throughout development. We also compared the protein distribution with the cellular distribution of the mRNA and of an antisense RNA.

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Acknowledgements

The authors would like to thank Dr. Steiner (University of Chicago) for kindly providing the rabbit antibody anti-HIG-1, Dr. Flavio Zolessi and Dr. Mónica Brauer for contributing with secondary antibodies, and Dr. Yanina Panzera for her assistance in the use of image processing software.

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Authors and Affiliations

  1. Sección Genética Evolutiva, Facultad de Ciencias, Universidad de la República, Iguá 4225, 11400, Montevideo, Uruguay

    Gabriela Bedó

  2. Departamento de Fisiología, Facultad de Medicina, Universidad de la República, General Flores 2125, 11800, Montevideo, Uruguay

    Patricia Lagos

  3. Sección Fisiología y Nutrición, Facultad de Ciencias, Universidad de la República, Iguá 4225, 11400, Montevideo, Uruguay

    Daniella Agrati

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  1. Gabriela Bedó
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  2. Patricia Lagos
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Correspondence to Gabriela Bedó.

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Bedó, G., Lagos, P. & Agrati, D. Temporal Distribution of Hig-1 (Hypoxia-Induced Gene 1) mRNA and Protein in Rat Spinal Cord: Changes During Postnatal Life. J Mol Neurosci 47, 666–673 (2012). https://doi.org/10.1007/s12031-012-9713-9

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  • DOI: https://doi.org/10.1007/s12031-012-9713-9

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