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Changes in the Expression of Pituitary Adenylate Cyclase-Activating Polypeptide in the Human Placenta during Pregnancy and Its Effects on the Survival of JAR Choriocarcinoma Cells

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Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP), a neuropeptide with survival-promoting actions, has been observed in endocrine organs and is thought to play a role in reproductive functions, including pregnancy. PACAP occurs in two forms, 27 and 38 amino acid residues, with PACAP38 being the predominant form in human tissues. In the present study, we determined the concentrations of PACAP38 and PACAP27 in first-trimester and full-term human placentas using radioimmunoassay. We found high levels of PACAP38 and lower levels of PACAP27 in different parts of the full-term human placenta. PACAP38 content increased in the placenta during pregnancy, both on the maternal side and on the fetal side. The effects of PACAP on the survival of JAR human choriocarcinoma cells were investigated using flow cytometry and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) cell viability assay in cells exposed to the widely used chemotherapeutic agent methotrexate (MTX). It was found that PACAP neither influenced the survival of JAR cytotrophoblast cells nor affected cellular response to the death-inducing effect of the chemotherapeutic agent MTX. The present observations further support the significance of PACAP in the human placenta. The observation that PACAP did not influence the effects of MTX may have future clinical importance, showing that PACAP does not decrease the effects of certain chemotherapeutic agents.

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Acknowledgements

This work was supported by the following grants: OTKA: K72592, F67830, 68469, CNK 78480, NFF-78790, F48543, K75965, Bolyai Scholarship, ETT278-04/2009, and University of Pecs Medical School Research Grant 2009.

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Authors and Affiliations

  1. Department of Anatomy, University of Pecs, Szigeti u 12, 7624, Pecs, Hungary

    R. Brubel, D. Reglodi, P. Kiss, A. Lubics, G. Horvath, D. Szogyi, E. Fonagy, J. Farkas & A. Tamas

  2. Department of Biochemistry and Medical Chemistry, University of Pecs, Pecs, Hungary

    A. Boronkai, B. Racz & Sz. Bellyei

  3. Department of Oncotherapy, University of Pecs, Pecs, Hungary

    A. Boronkai & Sz. Bellyei

  4. Department of Pharmacology and Pharmacotherapy, University of Debrecen, Debrecen, Hungary

    J. Nemeth

  5. Department of Medical Chemistry, University of Szeged, Szeged, Hungary

    G. Toth

  6. Department of Obstetrics and Gynecology, University of Pecs, Pecs, Hungary

    T. Varga & M. Koppan

  7. Department of Medical Microbiology and Immunology, University of Pecs, Pecs, Hungary

    A. Barakonyi & L. Szereday

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  1. R. Brubel
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  2. A. Boronkai
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  18. A. Tamas
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Correspondence to A. Tamas.

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Brubel, R., Boronkai, A., Reglodi, D. et al. Changes in the Expression of Pituitary Adenylate Cyclase-Activating Polypeptide in the Human Placenta during Pregnancy and Its Effects on the Survival of JAR Choriocarcinoma Cells. J Mol Neurosci 42, 450–458 (2010). https://doi.org/10.1007/s12031-010-9374-5

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